Construct: ORF TRCN0000492150

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018086.1_s317c1
Derived from:
ccsbBroadEn_00799
DNA Barcode:
TAACGAGTGACTGCATCACATTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HSP90AB1 (3326)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492150
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3326 HSP90AB1 heat shock protein 90 alpha... NM_001271969.1 100% 100%
2 human 3326 HSP90AB1 heat shock protein 90 alpha... NM_001271970.1 100% 100%
3 human 3326 HSP90AB1 heat shock protein 90 alpha... NM_001371238.1 100% 100%
4 human 3326 HSP90AB1 heat shock protein 90 alpha... NM_007355.4 100% 100%
5 human 3326 HSP90AB1 heat shock protein 90 alpha... NM_001271972.1 98.6% 98.6% 351_352ins30
6 human 3326 HSP90AB1 heat shock protein 90 alpha... NM_001271971.1 93.3% 93.3% 219_220ins144
7 human 664618 HSP90AB4P heat shock protein 90 alpha... NR_073415.2 60.3% (many diffs)
8 mouse 15516 Hsp90ab1 heat shock protein 90 alpha... NM_008302.3 89.6% 99.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2238
ORF length:
2172
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc tgaggaagtg caccatggag aggaggaggt ggagactttt gcctttcagg
121cagaaattgc ccaactcatg tccctcatca tcaatacctt ctattccaac aaggagattt
181tccttcggga gttgatctct aatgcttctg atgccttgga caagattcgc tatgagagcc
241tgacagaccc ttcgaagttg gacagtggta aagagctgaa aattgacatc atccccaacc
301ctcaggaacg taccctgact ttggtagaca caggcattgg catgaccaaa gctgatctca
361taaataattt gggaaccatt gccaagtctg gtactaaagc attcatggag gctcttcagg
421ctggtgcaga catctccatg attgggcagt ttggtgttgg cttttattct gcctacttgg
481tggcagagaa agtggttgtg atcacaaagc acaacgatga tgaacagtat gcttgggagt
541cttctgctgg aggttccttc actgtgcgtg ctgaccatgg tgagcccatt ggcaggggta
601ccaaagtgat cctccatctt aaagaagatc agacagagta cctagaagag aggcgggtca
661aagaagtagt gaagaagcat tctcagttca taggctatcc catcaccctt tatttggaga
721aggaacgaga gaaggaaatt agtgatgatg aggcagagga agagaaaggt gagaaagaag
781aggaagataa agatgatgaa gaaaaaccca agatcgaaga tgtgggttca gatgaggagg
841atgacagcgg taaggataag aagaagaaaa ctaagaagat caaagagaaa tacattgatc
901aggaagaact aaacaagacc aagcctattt ggaccagaaa ccctgatgac atcacccaag
961aggagtatgg agaattctac aagagcctca ctaatgactg ggaagaccac ttggcagtca
1021agcacttttc tgtagaaggt cagttggaat tcagggcatt gctatttatt cctcgtcggg
1081ctccctttga cctttttgag aacaagaaga aaaagaacaa catcaaactc tatgtccgcc
1141gtgtgttcat catggacagc tgtgatgagt tgataccaga gtatctcaat tttatccgtg
1201gtgtggttga ctctgaggat ctgcccctga acatctcccg agaaatgctc cagcagagca
1261aaatcttgaa agtcattcgc aaaaacattg ttaagaagtg ccttgagctc ttctctgagc
1321tggcagaaga caaggagaat tacaagaaat tctatgaggc attctctaaa aatctcaagc
1381ttggaatcca cgaagactcc actaaccgcc gccgcctgtc tgagctgctg cgctatcata
1441cctcccagtc tggagatgag atgacatctc tgtcagagta tgtttctcgc atgaaggaga
1501cacagaagtc catctattac atcactggtg agagcaaaga gcaggtggcc aactcagctt
1561ttgtggagcg agtgcggaaa cggggcttcg aggtggtata tatgaccgag cccattgacg
1621agtactgtgt gcagcagctc aaggaatttg atgggaagag cctggtctca gttaccaagg
1681agggtctgga gctgcctgag gatgaggagg agaagaagaa gatggaagag agcaaggcaa
1741agtttgagaa cctctgcaag ctcatgaaag aaatcttaga taagaaggtt gagaaggtga
1801caatctccaa tagacttgtg tcttcacctt gctgcattgt gaccagcacc TACGGCTGGA
1861CAGCCAATAT GGAGCGGATC ATGAAAGCCC AGGCACTTCG GGACAACTCC ACCATGGGCT
1921ATATGATGGC CAAAAAGCAC CTGGAGATCA ACCCTGACCA CCCCATTGTG GAGACGCTGC
1981GGCAGAAGGC TGAGGCCGAC AAGAATGATA AGGCAGTTAA GGACCTGGTG GTGCTGCTGT
2041TTGAAACCGC CCTGCTATCT TCTGGCTTTT CCCTTGAGGA TCCCCAGACC CACTCCAACC
2101GCATCTATCG CATGATCAAG CTAGGTCTAG GTATTGATGA AGATGAAGTG GCAGCAGAGG
2161AACCCAATGC TGCAGTTCCT GATGAGATCC CCCCTCTCGA GGGCGATGAG GATGCGTCTC
2221GCATGGAAGA AGTCGATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
2281TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
2341GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAACGAG TGACTGCATC
2401ACATTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)