Construct: ORF TRCN0000492150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018086.1_s317c1
- Derived from:
- ccsbBroadEn_00799
- DNA Barcode:
- TAACGAGTGACTGCATCACATTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSP90AB1 (3326)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492150
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3326 | HSP90AB1 | heat shock protein 90 alpha... | NM_001271969.1 | 100% | 100% | |
| 2 | human | 3326 | HSP90AB1 | heat shock protein 90 alpha... | NM_001271970.1 | 100% | 100% | |
| 3 | human | 3326 | HSP90AB1 | heat shock protein 90 alpha... | NM_001371238.1 | 100% | 100% | |
| 4 | human | 3326 | HSP90AB1 | heat shock protein 90 alpha... | NM_007355.4 | 100% | 100% | |
| 5 | human | 3326 | HSP90AB1 | heat shock protein 90 alpha... | NM_001271972.1 | 98.6% | 98.6% | 351_352ins30 |
| 6 | human | 3326 | HSP90AB1 | heat shock protein 90 alpha... | NM_001271971.1 | 93.3% | 93.3% | 219_220ins144 |
| 7 | human | 664618 | HSP90AB4P | heat shock protein 90 alpha... | NR_073415.2 | 60.3% | (many diffs) | |
| 8 | mouse | 15516 | Hsp90ab1 | heat shock protein 90 alpha... | NM_008302.3 | 89.6% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2238
- ORF length:
- 2172
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgaggaagtg caccatggag aggaggaggt ggagactttt gcctttcagg 121 cagaaattgc ccaactcatg tccctcatca tcaatacctt ctattccaac aaggagattt 181 tccttcggga gttgatctct aatgcttctg atgccttgga caagattcgc tatgagagcc 241 tgacagaccc ttcgaagttg gacagtggta aagagctgaa aattgacatc atccccaacc 301 ctcaggaacg taccctgact ttggtagaca caggcattgg catgaccaaa gctgatctca 361 taaataattt gggaaccatt gccaagtctg gtactaaagc attcatggag gctcttcagg 421 ctggtgcaga catctccatg attgggcagt ttggtgttgg cttttattct gcctacttgg 481 tggcagagaa agtggttgtg atcacaaagc acaacgatga tgaacagtat gcttgggagt 541 cttctgctgg aggttccttc actgtgcgtg ctgaccatgg tgagcccatt ggcaggggta 601 ccaaagtgat cctccatctt aaagaagatc agacagagta cctagaagag aggcgggtca 661 aagaagtagt gaagaagcat tctcagttca taggctatcc catcaccctt tatttggaga 721 aggaacgaga gaaggaaatt agtgatgatg aggcagagga agagaaaggt gagaaagaag 781 aggaagataa agatgatgaa gaaaaaccca agatcgaaga tgtgggttca gatgaggagg 841 atgacagcgg taaggataag aagaagaaaa ctaagaagat caaagagaaa tacattgatc 901 aggaagaact aaacaagacc aagcctattt ggaccagaaa ccctgatgac atcacccaag 961 aggagtatgg agaattctac aagagcctca ctaatgactg ggaagaccac ttggcagtca 1021 agcacttttc tgtagaaggt cagttggaat tcagggcatt gctatttatt cctcgtcggg 1081 ctccctttga cctttttgag aacaagaaga aaaagaacaa catcaaactc tatgtccgcc 1141 gtgtgttcat catggacagc tgtgatgagt tgataccaga gtatctcaat tttatccgtg 1201 gtgtggttga ctctgaggat ctgcccctga acatctcccg agaaatgctc cagcagagca 1261 aaatcttgaa agtcattcgc aaaaacattg ttaagaagtg ccttgagctc ttctctgagc 1321 tggcagaaga caaggagaat tacaagaaat tctatgaggc attctctaaa aatctcaagc 1381 ttggaatcca cgaagactcc actaaccgcc gccgcctgtc tgagctgctg cgctatcata 1441 cctcccagtc tggagatgag atgacatctc tgtcagagta tgtttctcgc atgaaggaga 1501 cacagaagtc catctattac atcactggtg agagcaaaga gcaggtggcc aactcagctt 1561 ttgtggagcg agtgcggaaa cggggcttcg aggtggtata tatgaccgag cccattgacg 1621 agtactgtgt gcagcagctc aaggaatttg atgggaagag cctggtctca gttaccaagg 1681 agggtctgga gctgcctgag gatgaggagg agaagaagaa gatggaagag agcaaggcaa 1741 agtttgagaa cctctgcaag ctcatgaaag aaatcttaga taagaaggtt gagaaggtga 1801 caatctccaa tagacttgtg tcttcacctt gctgcattgt gaccagcacc TACGGCTGGA 1861 CAGCCAATAT GGAGCGGATC ATGAAAGCCC AGGCACTTCG GGACAACTCC ACCATGGGCT 1921 ATATGATGGC CAAAAAGCAC CTGGAGATCA ACCCTGACCA CCCCATTGTG GAGACGCTGC 1981 GGCAGAAGGC TGAGGCCGAC AAGAATGATA AGGCAGTTAA GGACCTGGTG GTGCTGCTGT 2041 TTGAAACCGC CCTGCTATCT TCTGGCTTTT CCCTTGAGGA TCCCCAGACC CACTCCAACC 2101 GCATCTATCG CATGATCAAG CTAGGTCTAG GTATTGATGA AGATGAAGTG GCAGCAGAGG 2161 AACCCAATGC TGCAGTTCCT GATGAGATCC CCCCTCTCGA GGGCGATGAG GATGCGTCTC 2221 GCATGGAAGA AGTCGATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2281 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2341 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAACGAG TGACTGCATC 2401 ACATTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt