Transcript: Human NR_073415.2

Homo sapiens heat shock protein 90 alpha family class B member 4, pseudogene (HSP90AB4P), non-coding RNA.

Source:
NCBI, updated 2018-05-24
Taxon:
Homo sapiens (human)
Gene:
HSP90AB4P (664618)
Length:
3135
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_073415.2
NBCI Gene record:
HSP90AB4P (664618)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_073415.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000147710 GACAACTCTATGATGGGTTAT pLKO.1 2548 3UTR 100% 10.800 8.640 N HSP90AB6P n/a
2 TRCN0000159588 GAAGAGAAAGGTGAGAAAGAA pLKO.1 1095 3UTR 100% 5.625 2.813 Y HSP90AB2P n/a
3 TRCN0000138391 CGCCTGTAATCCTAGCACTTT pLKO.1 1652 3UTR 100% 4.950 2.475 Y DENND6A n/a
4 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 1811 3UTR 100% 10.800 5.400 Y SMIM11A n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_073415.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15457 pDONR223 0% 60.3% None (many diffs) n/a
2 ccsbBroad304_15457 pLX_304 0% 60.3% V5 (many diffs) n/a
3 ccsbBroadEn_00799 pDONR223 100% 60.3% None (many diffs) n/a
4 ccsbBroad304_00799 pLX_304 0% 60.3% V5 (many diffs) n/a
5 TRCN0000492150 TAACGAGTGACTGCATCACATTAC pLX_317 18.1% 60.3% V5 (many diffs) n/a
6 ccsbBroadEn_13764 pDONR223 100% 5.9% None (many diffs) n/a
7 ccsbBroad304_13764 pLX_304 0% 5.9% V5 (many diffs) n/a
8 TRCN0000465517 GTTGCCGATGGCTGCTATGAGATT pLX_317 92% 5.9% V5 (many diffs) n/a
Download CSV