Construct: ORF TRCN0000492153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000143.1_s317c1
- Derived from:
- ccsbBroadEn_16140
- DNA Barcode:
- CATGACTGTAGCGGTGCCCGGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN5P2 (260294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XM_005277604.5 | 49.3% | 32.6% | (many diffs) |
| 2 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168348.2 | 48.6% | 27.9% | (many diffs) |
| 3 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_018044.4 | 44.3% | 25.6% | (many diffs) |
| 4 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_148956.4 | 40.8% | 23.8% | (many diffs) |
| 5 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168347.3 | 40.5% | 23.6% | (many diffs) |
| 6 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XR_242255.5 | 34.8% | (many diffs) | |
| 7 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_033322.3 | 33.2% | (many diffs) | |
| 8 | human | 260294 | NSUN5P2 | NSUN5 pseudogene 2 | NR_033323.3 | 33.2% | 1_728del;1109_1110insGGTGAGAT;1324_1784del | |
| 9 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_104013.1 | 31.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggagctgctg gtgtttcccg cccagacaga tctgcatgaa cacccactgt 121 accgggccgg acacctcatt ctgcaggaca gggccagctg tctcccagcc atgctgctgg 181 acccccgcca ggctcccatg tcatggatgc ctgtgccacc ccaggcaata aagaccagtc 241 acttggctgc tcTTCTGAAG AACCAAGGGA AGATCTTTGC CTTTGACCTG GATGCCAGGC 301 GGCTGGCATC CATGGCCACG CTGCTGGCCT GGGCTGGCGT CTCCTGCTGT GAGCTGGCTG 361 AGGAGGACTT CCTGGCGGTC TCCCCCTTAG ATCCGCGCTA TCGTGAGGTC CACTATGTCC 421 TGCTGGATCC TTCCTGCAGT GGCTCGGGTG AGATGGTATG CCGAGCAGAC AGCTGGAGGA 481 GCCCGGGGCA GGGACACCTA GCCCGGTGCG TCTGCATGCC CTGGCAGGGT TCCAGCAGCG 541 AGCCCTGTGC CACGCGCTCA CTTTCCCTTC CCTGCAGCGG CTCGTCTACT CCATGTGCTC 601 CCTCTGCCAG GAGGAGAATG AAGACATGGT ACAAGATGCG CTGCAGCAGA ACCCGGGCGC 661 CTTCAGGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATGAC TGTAGCGGTG CCCGGACTAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt