Construct: ORF TRCN0000492159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019755.2_s317c1
- DNA Barcode:
- GCCAAATGTCGGTCCTCTCTTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MC2R (4158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4158 | MC2R | melanocortin 2 receptor | NM_000529.2 | 99.8% | 99.6% | 891_892insG |
| 2 | human | 4158 | MC2R | melanocortin 2 receptor | NM_001291911.1 | 99.8% | 99.6% | 891_892insG |
| 3 | human | 4158 | MC2R | melanocortin 2 receptor | XM_017025781.1 | 99.8% | 99.6% | 891_892insG |
| 4 | mouse | 17200 | Mc2r | melanocortin 2 receptor | NM_001271716.1 | 84% | 88.2% | (many diffs) |
| 5 | mouse | 17200 | Mc2r | melanocortin 2 receptor | NM_001271717.1 | 84% | 88.2% | (many diffs) |
| 6 | mouse | 17200 | Mc2r | melanocortin 2 receptor | NM_001301372.1 | 84% | 88.2% | (many diffs) |
| 7 | mouse | 17200 | Mc2r | melanocortin 2 receptor | NM_008560.3 | 84% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 963
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaagcacatt atcaactcgt atgaaaacat caacaacaca gcaagaaata 121 attccgactg tcctcgtgtg gttttgccgg aggagatatt tttcacaatt tccattgttg 181 gagttttgga gaatctgatc gtcctgctgg ctgtgttcaa gaataagaat ctccaggcac 241 ccatgtactt tttcatctgt agcttggcca tatctgatat gctgggcagc ctatataaga 301 tcttggaaaa tatcctgatc atattgagaa acatgggcta tctcaagcca cgtggcagtt 361 ttgaaaccac agccgatgac atcatcgact ccctgtttgt cctctccctg cttggctcca 421 tcttcagcct gtctgtgatt gctgcggacc gctacatcac catcttccac gcactgcggt 481 accacagcat cgtgaccatg cgccgcactg tggtggtgct tacggtcatc tggacgttct 541 gcacggggac tggcatcacc atggtgatct tctcccatca tgtgcccaca gtgatcaccT 601 TCACGTCGCT GTTCCCGCTG ATGCTGGTCT TCATCCTGTG CCTCTATGTG CACATGTTCC 661 TGCTGGCTCG ATCCCACACC AGGAAGATCT CCACCCTCCC CAGAGCCAAC ATGAAAGGGG 721 CCATCACACT GACCATCCTG CTCGGGGTCT TCATCTTCTG CTGGGCCCCC TTTGTGCTTC 781 ATGTCCTCTT GATGACATTC TGCCCAAGTA ACCCCTACTG CGCCTGCTAC ATGTCTCTCT 841 TCCAGGTGAA CGGCATGTTG ATCATGTGCA ATGCCGTCAT TGACCCCTTC ATATATGCCT 901 TCCGGAGCCC AGAGCTCAGG GACGCATTCA AAAAGATGAT CTTCTGCAGC AGGTACTGGG 961 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAGCCAA ATGTCGGTCC TCTCTTATAA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt