Construct: ORF TRCN0000492163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011536.2_s317c1
- Derived from:
- ccsbBroadEn_05089
- DNA Barcode:
- CTTACAATGATTATCCAATAAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGR3 (155465)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 155465 | AGR3 | anterior gradient 3, protei... | NM_176813.5 | 100% | 100% | |
| 2 | human | 155465 | AGR3 | anterior gradient 3, protei... | XM_005249627.2 | 100% | 100% | |
| 3 | human | 155465 | AGR3 | anterior gradient 3, protei... | XM_011515152.2 | 90.3% | 90.3% | 450_451ins48 |
| 4 | mouse | 403205 | Agr3 | anterior gradient 3 | NM_207531.3 | 85.3% | 90.9% | (many diffs) |
| 5 | mouse | 403205 | Agr3 | anterior gradient 3 | XM_006515142.3 | 53.4% | 57.2% | (many diffs) |
| 6 | mouse | 403205 | Agr3 | anterior gradient 3 | XM_006515143.1 | 53.4% | 57.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gctacactca gctttgggtc tctgcctctt actcgtcaca gtttcttcca 121 accttgccat tgcaataaaa aaggaaaaga ggcctcctca gacactctca agaggatggg 181 gagatgacat cacttGGGTA CAAACTTATG AAGAAGGTCT CTTTTATGCT CAAAAAAGTA 241 AGAAGCCATT AATGGTTATT CATCACCTGG AGGATTGTCA ATACTCTCAA GCACTAAAGA 301 AAGTATTTGC CCAAAATGAA GAAATACAAG AAATGGCTCA GAATAAGTTC ATCATGCTAA 361 ACCTTATGCA TGAAACCACT GATAAGAATT TATCACCTGA TGGGCAATAT GTGCCTAGAA 421 TCATGTTTGT AGACCCTTCT TTAACAGTTA GAGCTGACAT AGCTGGAAGA TACTCTAACA 481 GATTGTACAC ATATGAGCCT CGGGATTTAC CCCTATTGAT AGAAAACATG AAGAAAGCAT 541 TAAGACTTAT TCAGTCAGAG CTATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TTACAATGAT 721 TATCCAATAA ACTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att