Construct: ORF TRCN0000492174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020333.2_s317c1
- DNA Barcode:
- ATGCAGAACATGCAAACTCACTCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FRK (2444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492174
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2444 | FRK | fyn related Src family tyro... | NM_002031.3 | 100% | 100% | |
| 2 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266880.4 | 100% | 100% | |
| 3 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266881.2 | 100% | 100% | |
| 4 | human | 2444 | FRK | fyn related Src family tyro... | XM_005266882.4 | 100% | 100% | |
| 5 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535653.2 | 100% | 100% | |
| 6 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535654.2 | 100% | 100% | |
| 7 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535655.2 | 100% | 100% | |
| 8 | human | 2444 | FRK | fyn related Src family tyro... | XM_017010645.1 | 100% | 100% | |
| 9 | human | 2444 | FRK | fyn related Src family tyro... | XM_011535656.2 | 77.5% | 77.2% | 0_1ins338;2delT;5_6insCC |
| 10 | mouse | 14302 | Frk | fyn-related kinase | NM_001159544.1 | 86% | 88.2% | (many diffs) |
| 11 | mouse | 14302 | Frk | fyn-related kinase | NM_010237.3 | 86% | 88.2% | (many diffs) |
| 12 | mouse | 14302 | Frk | fyn-related kinase | XM_006512534.3 | 86% | 88.2% | (many diffs) |
| 13 | mouse | 14302 | Frk | fyn-related kinase | XM_006512535.3 | 86% | 88.2% | (many diffs) |
| 14 | mouse | 14302 | Frk | fyn-related kinase | XM_006512536.3 | 86% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1587
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcaac atctgtcaga ggctctggga gtacctagaa ccctatctcc 121 cctgtttgtc cacggaggca gacaagtcaa ccgtgattga aaatccaggg gccctttgct 181 ctccccagtc acagaggcat ggccactact ttgtggcttt gtttgattac caggctcgga 241 ctgctgagga cttgagcttc cgagcaggtg acaaacttca agttctggac actttgcatg 301 agggctggtg gtttgccaga cacttggaga aaagacgaga tggctccagt cagcaactac 361 aaggctatat tccttctaac tacgtggctg aggacagaag cctacaggca gagccgtggt 421 tctttggagc aatcggaaga tcagatgcag agaaacaact attatattca gaaaacaaga 481 ccggttcctt tctaatcaga gaaagtgaaa gccaaaaagg agaattctct ctttcagttt 541 tagatggagc agttgtaaaa cactacagaa ttaaaagact ggatgaaggg ggattttttc 601 tcacgcgaag aagaatcttt tcaacactga acgaatttgt gagccactac accaagacaa 661 gtgacggcct gtgtgtcaag ctggggaaac catgcttaaa gatccaggtc ccagctccat 721 ttgatttgtc gtataaaacc gtggaccaat gggagataga ccgcaactcc atacagcttc 781 tgaagcgatt gggatctggt cagtttggcg aagtatggga aggtctgtgg aacaatacca 841 ctccagtagc agtgaaaaca ttaaaaccag gttcaatgga tccaaatgac ttcctgaggg 901 aggcacagat aatgaagaac ctaagacatc caaagcttat ccagctttat gctgtttgca 961 ctttagaaga tccaatttat attattacag agttgatgag acatggaagt ctgcaagaat 1021 atctccaaaa tgacactgga tcaaaaatcc atctgactca acaggtagac atggcggcac 1081 aggttgcctc tggaatggcc tatctggagt ctcggaacta cattcacaga gatctggctg 1141 ccagaaatgt cctcgttggt gaacataata tcTACAAAGT AGCAGATTTT GGACTTGCCA 1201 GAGTTTTTAA GGTAGATAAT GAAGACATCT ATGAATCTAG ACACGAAATA AAGCTGCCGG 1261 TGAAGTGGAC TGCGCCCGAA GCCATTCGTA GTAATAAATT CAGCATTAAG TCCGATGTAT 1321 GGTCATTTGG AATCCTTCTT TATGAAATCA TTACTTATGG CAAAATGCCT TACAGTGGTA 1381 TGACAGGTGC CCAGGTAATC CAGATGTTGG CTCAAAACTA TAGACTTCCG CAACCATCCA 1441 ACTGTCCACA GCAATTTTAC AACATCATGT TGGAGTGCTG GAATGCAGAG CCTAAGGAAC 1501 GACCTACATT TGAGACACTG CGTTGGAAAC TTGAAGACTA TTTTGAAACA GACTCTTCAT 1561 ATTCAGATGC AAATAACTTC ATAAGATGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATGCA 1741 GAACATGCAA ACTCACTCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt