Construct: ORF TRCN0000492199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021852.2_s317c1
- DNA Barcode:
- ACCGGCCTATTCCTTCTGTTGGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HSPA1A (3303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3303 | HSPA1A | heat shock protein family A... | NM_005345.6 | 99.8% | 100% | 222T>C;1710G>T;1920A>G |
| 2 | human | 3304 | HSPA1B | heat shock protein family A... | NM_005346.6 | 99.8% | 100% | 222T>C;1860C>G;1905T>C |
| 3 | human | 3305 | HSPA1L | heat shock protein family A... | NM_005527.4 | 81% | 88.1% | (many diffs) |
| 4 | mouse | 193740 | Hspa1a | heat shock protein 1A | NM_010479.2 | 91.6% | 95.3% | (many diffs) |
| 5 | mouse | 15511 | Hspa1b | heat shock protein 1B | NM_010478.2 | 91.5% | 95.1% | (many diffs) |
| 6 | mouse | 15482 | Hspa1l | heat shock protein 1-like | NM_013558.2 | 81.9% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1992
- ORF length:
- 1923
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccaaagcc gcggcgatcg gcatcgacct gggcaccacc tactcctgcg 121 tgggggtgtt ccaacacggc aaggtggaga tcatcgccaa cgaccagggc aaccgcacca 181 cccccagcta cgtggccttc acggacaccg agcggctcat cggggatgcg gccaagaacc 241 aggtggcgct gaacccgcag aacaccgtgt ttgacgcgaa gcggctgatc ggccgcaagt 301 tcggcgaccc ggtggtgcag tcggacatga agcactggcc tttccaggtg atcaacgacg 361 gagacaagcc caaggtgcag gtgagctaca agggggagac caaggcattc taccccgagg 421 agatctcgtc catggtgctg accaagatga aggagatcgc cgaggcgtac ctgggctacc 481 cggtgaccaa cgcggtgatc accgtgccgg cctacttcaa cgactcgcag cgccaggcca 541 ccaaggatgc gggtgtgatc gcggggctca acgtgctgcg gatcatcaac gagcccacgg 601 ccgccgccat cgcctacggc ctggacagaa cgggcaaggg ggagcgcaac gtgctcatct 661 ttgacctggg cgggggcacc ttcgacgtgt ccatcctgac gatcgacgac ggcatcttcg 721 aggtgaaggc cacggccggg gacacccacc tgggtgggga ggactttgac aacaggctgg 781 tgaaccactt cgtggaggag ttcaagagaa aacacaagaa ggacatcagc cagaacaagc 841 gagccgtgag gcggctgcgc accgcctgcg agagggccaa gaggaccctg tcgtccagca 901 cccaggccag cctggagatc gactccctgt ttgagggcat cgacttctac acgtccatca 961 ccagggcgag gttcgaggag ctgtgctccg acctgttccg aagcaccctg gagcccgtgg 1021 agaaggctct gcgcgacgcc aagctggaca aggcccagat tcacgacctg gtcctggtcg 1081 ggggctccac ccgcatcccc aaggtgcaga agctgctgca ggacttcttc aacgggcgcg 1141 acctgaacaa gagcatcaac cccgacgagg ctgtggccta cggggcggcg gtgcaggcgg 1201 ccatcctgat gggggacaag tccgagaacg tgcaggacct gctgctgctg gacgtggctc 1261 ccctgtcgct ggggctggag acggccggag gcgtgatgac tgccctgatc aagcgcaact 1321 ccaccatccc caccaagcag acgcagatct tcaccaccta ctccgacaac caacccgggg 1381 tgctgatcca ggtgtacgag ggcgagaggg ccatgacgaa agacaacaat ctgttggggc 1441 gcttcgagct gagcggcatc cctccggccc ccaggggcgt gccccagatc gaggtgacct 1501 tcgacatcga tgccaacggc atcctgaacg tcacggccac ggacaagagc accggcaagg 1561 ccaacaagat caccatcacc aacgacaagg gccgcctgag CAAGGAGGAG ATCGAGCGCA 1621 TGGTGCAGGA GGCGGAGAAG TACAAAGCGG AGGACGAGGT GCAGCGCGAG AGGGTGTCAG 1681 CCAAGAACGC CCTGGAGTCC TACGCCTTCA ACATGAAGAG CGCCGTGGAG GATGAGGGGC 1741 TCAAGGGCAA GATCAGCGAG GCGGACAAGA AGAAGGTTCT GGACAAGTGT CAAGAGGTCA 1801 TCTCGTGGCT GGACGCCAAC ACCTTGGCCG AGAAGGACGA GTTTGAGCAC AAGAGGAAGG 1861 AGCTGGAGCA GGTGTGTAAC CCCATCATCA GCGGACTGTA CCAGGGTGCC GGTGGTCCCG 1921 GGCCTGGGGG CTTCGGGGCT CAGGGTCCCA AGGGAGGGTC TGGGTCAGGC CCCACCATTG 1981 AGGAGGTGGA TTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2041 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2101 TTTCGATTTC TTGGCTTTAT AATCTTGTGG AAAGGACGAA CCGGCCTATT CCTTCTGTTG 2161 GGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att