Construct: ORF TRCN0000492215
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021289.2_s317c1
- DNA Barcode:
- TGCTACTGTCTCCCGTGGCGCAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FUT10 (84750)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492215
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84750 | FUT10 | fucosyltransferase 10 | NM_032664.3 | 28.6% | 27.5% | (many diffs) |
| 2 | human | 84750 | FUT10 | fucosyltransferase 10 | XM_011544681.3 | 27.2% | 26.1% | (many diffs) |
| 3 | human | 84750 | FUT10 | fucosyltransferase 10 | XM_011544680.3 | 26.3% | 25.2% | (many diffs) |
| 4 | human | 84750 | FUT10 | fucosyltransferase 10 | XM_011544679.3 | 26.2% | 25.1% | (many diffs) |
| 5 | human | 84750 | FUT10 | fucosyltransferase 10 | XM_011544677.3 | 24.4% | 23.4% | (many diffs) |
| 6 | human | 84750 | FUT10 | fucosyltransferase 10 | XM_011544676.3 | 22.7% | 21.8% | (many diffs) |
| 7 | human | 84750 | FUT10 | fucosyltransferase 10 | XM_011544678.2 | 19.4% | 18.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 498
- ORF length:
- 429
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg taCAAAAAAG 61 CAGGCACCAT GGTGCGGATT CAGAGGAGGA AGCTTTTGGC ATCTTGCCTG TGCGTCACAG 121 CCACCGTCTT TCTGCTTGTC ACACTCCAGG TCATGGTTGA GCTGGGGAAG TTTGAAAGGA 181 AGGAGTTTAA AAGTTCCAGT TTGCAAGATG GACATACAAA AATGGAGGAA GCACCTACGC 241 ATCTTAATTC ATTTCTTAAG AAAGAAGGAT TGACCTTCAA CAGGAAAAGA AAATGGGAAT 301 TGGACAGCTA CCCCATTATG CTCTGGTGGT CCCCGCTGAC GGGGGAGACT GGGAGGTTAG 361 GCCAATGTGG AGCAGATGCT TGTTTCTTCA CCATCAACCG GACCTACCTC CATCATCACA 421 TGACCAAAGC ATTCCTCTTC TATGGTAAGC AGGACTTTCG CCTTTCTCCC TTGTTTGCTG 481 TGGTCTTCCT CCAAAGCTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCT ACTGTCTCCC 661 GTGGCGCAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt