Construct: ORF TRCN0000492238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014317.2_s317c1
- Derived from:
- ccsbBroadEn_01198
- DNA Barcode:
- GTATCCGACTGATATCGTAATGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGA (5277)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492238
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5277 | PIGA | phosphatidylinositol glycan... | NM_002641.3 | 100% | 100% | |
| 2 | human | 5277 | PIGA | phosphatidylinositol glycan... | XM_011545539.2 | 51.9% | 51.2% | (many diffs) |
| 3 | human | 5277 | PIGA | phosphatidylinositol glycan... | NM_020473.3 | 51.5% | 50.8% | (many diffs) |
| 4 | human | 5277 | PIGA | phosphatidylinositol glycan... | NR_033835.1 | 29.5% | 1_116del;455_456ins374;1195_3270del | |
| 5 | human | 5277 | PIGA | phosphatidylinositol glycan... | NR_033836.1 | 23.8% | (many diffs) | |
| 6 | mouse | 18700 | Piga | phosphatidylinositol glycan... | NM_011081.2 | 87.4% | 87.6% | (many diffs) |
| 7 | mouse | 18700 | Piga | phosphatidylinositol glycan... | XM_017318428.1 | 87.4% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1518
- ORF length:
- 1452
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgtagagga ggagctggga atggccaccg tgcctcagct acactctctc 121 gggttagccc tggaagtctt tacacatgta gaacccgtac ccataatata tgcatggtat 181 ctgacttttt ctacccaaat atgggaggcg tggaaagcca catttaccag ctctctcagt 241 gcctgattga aagagggcat aaggttataa ttgtcaccca tgcttatgga aatcgaaaag 301 gcatccgtta cctcaccagt ggcctcaaag tctattactt gcctctgaaa gtcatgtaca 361 accagtctac agccacgacc ctctttcaca gtctgccatt gctcaggtac atatttgttc 421 gggagagagt cacgataatc cattcacata gttctttttc tgctatggcc catgatgctc 481 tcttccacgc caagacaatg gggcttcaga cagtcttcac ggaccattcc ctttttggat 541 ttgctgatgt cagctcggtg cttacaaaca agcttctaac cgtgtctctt tgtgatacaa 601 accacatcat ttgtgtgtct tatactagta aggaaaatac tgtactaaga gcagcactga 661 atcctgaaat agtgtccgtc attcctaatg ctgtagatcc tactgacttc actccagacc 721 catttagaag gcatgatagt ataactattg ttgttgtcag cagacttgtt tacagaaaag 781 ggatcgattt gcttagtggt ataatacctg aactctgtca gaaatatcca gatttaaatt 841 tcataattgg aggagaggga ccaaagagaa tcattttgga agaagttcgg gaaagatacc 901 agctgcatga cagggtgcgt cttttgggag ctttagaaca caaggatgtt agaaatgtct 961 tagttcaagg acatattttt ctgaatacct cccttactga agcattctgc atggcgatcg 1021 tggaagcagc cagttgtggt ttacaggttg taagtaccag agttggtgga attcctgagg 1081 tgcttccaga aaaccttatt attttatgtg agccttcagt aaaatctttg tgtgaaggat 1141 tggaaaaggc tattttccaa ctgaagtcag ggacattgcc agctccagaa aacatccata 1201 acatagtaaa gactttctac acctGGAGGA ATGTTGCAGA AAGAACTGAA AAGGTATATG 1261 ACCGGGTATC AGTGGAAGCT GTGTTGCCAA TGGACAAACG ACTGGACAGA CTTATTTCTC 1321 ACTGCGGCCC AGTAACAGGC TACATCTTTG CTTTGTTGGC AGTTTTCAAC TTCCTCTTCC 1381 TCATTTTCTT GAGATGGATG ACTCCAGATT CTATCATTGA TGTTGCAATA GATGCCACTG 1441 GGCCACGGGG TGCCTGGACT AATAACTATT CTCACAGTAA AAGAGGGGGT GAGAATAATG 1501 AGATATCTGA AACCAGGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTATCCG ACTGATATCG 1681 TAATGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt