Construct: ORF TRCN0000492240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013075.1_s317c1
- Derived from:
- ccsbBroadEn_05411
- DNA Barcode:
- TAGTCCACCTGCGCCGTTGACAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSMEM1 (286006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492240
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286006 | LSMEM1 | leucine rich single-pass me... | NM_001134468.1 | 100% | 100% | |
| 2 | human | 286006 | LSMEM1 | leucine rich single-pass me... | NM_182597.3 | 100% | 100% | |
| 3 | human | 286006 | LSMEM1 | leucine rich single-pass me... | XM_011516074.2 | 100% | 100% | |
| 4 | human | 286006 | LSMEM1 | leucine rich single-pass me... | XM_011516075.2 | 100% | 100% | |
| 5 | human | 286006 | LSMEM1 | leucine rich single-pass me... | XM_011516076.2 | 100% | 100% | |
| 6 | human | 286006 | LSMEM1 | leucine rich single-pass me... | XM_017012028.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 462
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactcattct tcccaggaca ctggttcttG TGGCATTCAG GAAGATGGAA 121 AGCTTTATGT GGTGGATTCC ATAAATGACT TAAACAAACT AAACCTCTGT CCAGCCGGAT 181 CGCAGCATCT GTTCCCTCTA GAGGACAAAA TCCCAGTCCT TGGCACAAAC TCAGGAAATG 241 GAAGCCGGAG TCTGTTTTTT GTGGGGCTGC TAATTGTGCT GATTGTCAGC CTGGCACTGG 301 TTTTTTTCGT GATATTTCTA ATAGTTCAAA CTGGAAACAA GATGGATGAT GTGTCAAGAA 361 GACTAACAGC TGAAGGAAAA GACATAGATG ATCTTAAGAG AATCAATAAC ATGATCGTAA 421 AGCGACTCAA CCAACTCAAC CAACTGGACT CTGAACAAAA CTTGCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGATAG TCCACCTGCG CCGTTGACAG GACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t