Transcript: Human XM_011516075.2

PREDICTED: Homo sapiens leucine rich single-pass membrane protein 1 (LSMEM1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LSMEM1 (286006)
Length:
4040
CDS:
2532..2927

Additional Resources:

NCBI RefSeq record:
XM_011516075.2
NBCI Gene record:
LSMEM1 (286006)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011516075.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000167483 GTGGATTCCATAAATGACTTA pLKO.1 2595 CDS 100% 4.950 6.930 N LSMEM1 n/a
2 TRCN0000435966 TCGTGATATTTCTAATAGTTC pLKO_005 2770 CDS 100% 4.950 6.930 N LSMEM1 n/a
3 TRCN0000412957 CTCATGCACCTTTACAGATAT pLKO_005 3301 3UTR 100% 13.200 9.240 N LSMEM1 n/a
4 TRCN0000167388 GCTGATTGATTTGGAAGAAAT pLKO.1 3702 3UTR 100% 13.200 9.240 N LSMEM1 n/a
5 TRCN0000428576 GGCATTCAGGAAGATGGAAAG pLKO_005 2565 CDS 100% 6.000 4.200 N LSMEM1 n/a
6 TRCN0000167621 GATGATGTGTCAAGAAGACTA pLKO.1 2808 CDS 100% 4.950 3.465 N LSMEM1 n/a
7 TRCN0000168459 GCTGCTAATTGTGCTGATTGT pLKO.1 2729 CDS 100% 4.950 3.465 N LSMEM1 n/a
8 TRCN0000167484 GAGAATCAATAACATGATCGT pLKO.1 2861 CDS 100% 2.640 1.848 N LSMEM1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011516075.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05411 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05411 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000492240 TAGTCCACCTGCGCCGTTGACAGG pLX_317 89.6% 100% 100% V5 n/a
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