Construct: ORF TRCN0000492241
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019085.2_s317c1
- DNA Barcode:
- TTATACGGTATATTGTTACCGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDR (3791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492241
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3791 | KDR | kinase insert domain receptor | NM_002253.3 | 99.9% | 99.8% | 889G>A;4068_4069insG |
| 2 | mouse | 16542 | Kdr | kinase insert domain protei... | NM_010612.2 | 84.6% | 85.6% | (many diffs) |
| 3 | mouse | 16542 | Kdr | kinase insert domain protei... | XM_011240817.2 | 73.2% | 74.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4143
- ORF length:
- 4071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagagc aaggtgctgc tggccgtcgc cctgtggctc tgcgtggaga 121 cccgggccgc ctctgtgggt ttgcctagtg tttctcttga tctgcccagg ctcagcatac 181 aaaaagacat acttacaatt aaggctaata caactcttca aattacttgc aggggacaga 241 gggacttgga ctggctttgg cccaataatc agagtggcag tgagcaaagg gtggaggtga 301 ctgagtgcag cgatggcctc ttctgtaaga cactcacaat tccaaaagtg atcggaaatg 361 acactggagc ctacaagtgc ttctaccggg aaactgactt ggcctcggtc atttatgtct 421 atgttcaaga ttacagatct ccatttattg cttctgttag tgaccaacat ggagtcgtgt 481 acattactga gaacaaaaac aaaactgtgg tgattccatg tctcgggtcc atttcaaatc 541 tcaacgtgtc actttgtgca agatacccag aaaagagatt tgttcctgat ggtaacagaa 601 tttcctggga cagcaagaag ggctttacta ttcccagcta catgatcagc tatgctggca 661 tggtcttctg tgaagcaaaa attaatgatg aaagttacca gtctattatg tacatagttg 721 tcgttgtagg gtataggatt tatgatgtgg ttctgagtcc gtctcatgga attgaactat 781 ctgttggaga aaagcttgtc ttaaattgta cagcaagaac tgaactaaat gtggggattg 841 acttcaactg ggaataccct tcttcgaagc atcagcataa gaaacttgta aaccgagacc 901 taaaaaccca gtctgggagt gagatgaaga aatttttgag caccttaact atagatggta 961 taacccggag tgaccaagga ttgtacacct gtgcagcatc cagtgggctg atgaccaaga 1021 agaacagcac atttgtcagg gtccatgaaa aaccttttgt tgcttttgga agtggcatgg 1081 aatctctggt ggaagccacg gtgggggagc gtgtcagaat ccctgcgaag taccttggtt 1141 acccaccccc agaaataaaa tggtataaaa atggaatacc ccttgagtcc aatcacacaa 1201 ttaaagcggg gcatgtactg acgattatgg aagtgagtga aagagacaca ggaaattaca 1261 ctgtcatcct taccaatccc atttcaaagg agaagcagag ccatgtggtc tctctggttg 1321 tgtatgtccc accccagatt ggtgagaaat ctctaatctc tcctgtggat tcctaccagt 1381 acggcaccac tcaaacgctg acatgtacgg tctatgccat tcctcccccg catcacatcc 1441 actggtattg gcagttggag gaagagtgcg ccaacgagcc cagccaagct gtctcagtga 1501 caaacccata cccttgtgaa gaatggagaa gtgtggagga cttccaggga ggaaataaaa 1561 ttgaagttaa taaaaatcaa tttgctctaa ttgaaggaaa aaacaaaact gtaagtaccc 1621 ttgttatcca agcggcaaat gtgtcagctt tgtacaaatg tgaagcggtc aacaaagtcg 1681 ggagaggaga gagggtgatc tccttccacg tgaccagggg tcctgaaatt actttgcaac 1741 ctgacatgca gcccactgag caggagagcg tgtctttgtg gtgcactgca gacagatcta 1801 cgtttgagaa cctcacatgg tacaagcttg gcccacagcc tctgccaatc catgtgggag 1861 agttgcccac acctgtttgc aagaacttgg atactctttg gaaattgaat gccaccatgt 1921 tctctaatag cacaaatgac attttgatca tggagcttaa gaatgcatcc ttgcaggacc 1981 aaggagacta tgtctgcctt gctcaagaca ggaagaccaa gaaaagacat tgcgtggtca 2041 ggcagctcac agtcctagag cgtgtggcac ccacgatcac aggaaacctg gagaatcaga 2101 cgacaagtat tggggaaagc atcgaagtct catgcacggc atctgggaat ccccctccac 2161 agatcatgtg gtttaaagat aatgagaccc ttgtagaaga ctcaggcatt gtattgaagg 2221 atgggaaccg gaacctcact atccgcagag tgaggaagga ggacgaaggc ctctacacct 2281 gccaggcatg cagtgttctt ggctgtgcaa aagtggaggc atttttcata atagaaggtg 2341 cccaggaaaa gacgaacttg gaaatcatta ttctagtagg cacggcggtg attgccatgt 2401 tcttctggct acttcttgtc atcatcctac ggaccgttaa gcgggccaat ggaggggaac 2461 tgaagacagg ctacttgtcc atcgtcatgg atccagatga actcccattg gatgaacatt 2521 gtgaacgact gccttatgat gccagcaaat gggaattccc cagagaccgg ctgaagctag 2581 gtaagcctct tggccgtggt gcctttggcc aagtgattga agcagatgcc tttggaattg 2641 acaagacagc aacttgcagg acagtagcag tcaaaatgtt gaaagaagga gcaacacaca 2701 gtgagcatcg agctctcatg tctgaactca agatcctcat tcatattggt caccatctca 2761 atgtggtcaa ccttctaggt gcctgtacca agccaggagg gccactcatg gtgattgtgg 2821 aattctgcaa atttggaaac ctgtccactt acctgaggag caagagaaat gaatttgtcc 2881 cctacaagac caaaggggca cgattccgtc aagggaaaga ctacgttgga gcaatccctg 2941 tggatctgaa acggcgcttg gacagcatca ccagtagcca gagctcagcc agctctggat 3001 ttgtggagga gaagtccctc agtgatgtag aagaagagga agctcctgaa gatctgtata 3061 aggacttcct gaccttggag catctcatct gttacagctt ccaagtggct aagggcatgg 3121 agttcttggc atcgcgaaag tgtatccaca gggacctggc ggcacgaaat atcctcttat 3181 cggagaagaa cgtggttaaa atctgtgact ttggcttggc ccgggatatt tataaagatc 3241 cagattatgt cagaaaagga gatgctcgcc tccctttgaa atggatggcc ccagaaacaa 3301 tttttgacag agtgtacaca atccagagtg acgtctggtc ttttggtgtt ttgctgtggg 3361 aaatattttc cttaggtgct tctccatatc ctggggtaaa gattgatgaa gaattttgta 3421 ggcgattgaa agaaggaact agaatgaggg cccctgatta tactacacca gaaatgtacc 3481 agaccatgct ggactgctgg cacggggagc ccagtcagag acccacgttt tcagagttgg 3541 tggaacattt gggaaatctc ttgcaagcta atgctcagca ggatggcaaa gactacattg 3601 ttcttccgat atcagagact ttgagcatgg aagaggattc tggactctct ctgcctacct 3661 cacctgtttc ctgtatggag gaggaggaag tatgtgaccc caaattccat tatgacaaca 3721 cagcaggaat cagtcagtat ctgcagaaca gtaagcgaaa gagccggcct gtgagtgtaa 3781 aaacatttga agatatcccg ttagaagaac cagaagtaaa agtaatccca gatgacaacc 3841 agacggacag tggtatggtt cttgcctcag aagagctgaa aactttggaa gacagaacca 3901 aattatctcc atcttttggt ggaatggtgc ccagcaaaag cagggagtcT GTGGCATCTG 3961 AAGGCTCAAA CCAGACAAGC GGCTACCAGT CCGGATATCA CTCCGATGAC ACAGACACCA 4021 CCGTGTACTC CAGTGAGGAA GCAGAACTTT TAAAGCTGAT AGAGATTGGA GTGCAAACCG 4081 GTAGCACAGC CCAGATTCTC CAGCCTGACT CGGGGACCAC ACTGAGCTCT CCTCCTGTTG 4141 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 4201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 4261 TTTATATATC TTGTGGAAAG GACGATTATA CGGTATATTG TTACCGGTGA CGCGTTAAGT 4321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt