Construct: ORF TRCN0000492250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004086.2_s317c1
- Derived from:
- ccsbBroadEn_15483
- DNA Barcode:
- CTGGCTCGGCCATACATTTCTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3E (3646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3646 | EIF3E | eukaryotic translation init... | NM_001568.3 | 88.9% | 88.9% | 1_147del |
| 2 | human | 3646 | EIF3E | eukaryotic translation init... | XM_017013398.2 | 86.3% | 84.8% | (many diffs) |
| 3 | mouse | 16341 | Eif3e | eukaryotic translation init... | NM_008388.2 | 80.8% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1254
- ORF length:
- 1188
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt agactttgct atggatgtat acaaaaacct ttattctgat gatattcctc 121 atgctttgag agagaaaaga accacagtgg ttgcacaact gaaacagctt caggcagaaa 181 cagaaccaat tgtgaagatg tttgaagatc cagaaactac aaggcaaatg cagtcaacca 241 gggatggtag gatgctcttt gactacctgg cggacaagca tggttttagg caggaatatt 301 tagatacact ctacagatat gcaaaattcc agtacgaatg tgggaattac tcaggagcag 361 cagaatatct ttattttttt agagtgctgg ttccagcaac agatagaaat gctttaagtt 421 cactctgggg aaagctggcc tctgaaatct taatgcagaa ttgggatgca gccatggaag 481 accttacacg gttaaaagag accatagata ataattctgt gagttctcca cttcagtctc 541 ttcagcagag aacatggctc attcactggt ctctgtttgt tttcttcaat caccccaaag 601 gtcgcgataa tattattgac ctcttccttt atcagccaca atatcttaat gcaattcaga 661 caatgtgtcc acacattctt cgctatttga ctacagcagt cataacaaac aaggatgttc 721 gaaaacgtcg gcaggttcta aaagatctag ttaaagttat tcaacaggag tcttacacat 781 ataaagaccc aattacagaa tttgttgaat gtttatatgt taactttgac tttgatgggg 841 ctcagaaaaa gctgagggaa tgtgaatcag tgcttgtgaa tgacttcttc ttggtggctt 901 gtcttgagga tttcattgaa aatgcccgtc tcttcatatt tgagactttc tgtcgcatcc 961 accagtgtat cagcattaac atgttggcag ataaattgaa catgactcca gaagaagctg 1021 aaaggtggat tgtaaatttg attagaaatg caagactgga tgccaagatt GATTCTAAAT 1081 TAGGTCATGT GGTTATGGGT AACAATGCAG TCTCACCCTA TCAGCAAGTG ATTGAAAAGA 1141 CCAAAAGCCT TTCCTTTAGA AGCCAGATGT TGGCCATGAA TATTGAGAAG AAACTTAATC 1201 AGAATAGCAG GTCAGAGGCT CCTAACTGGG CAACTCAAGA TTCTGGCTTC TACTGCCCAA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATCTTGTGG AAAGGACGAC TGGCTCGGCC ATACATTTCT TAAACGCGTT AAGTCgacaa 1441 tcaacctctg gattacaaaa tttgtgaaag att