Construct: ORF TRCN0000492264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019263.2_s317c1
- DNA Barcode:
- TTCACCGCCCCCTTGCGGCTGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRF3 (165082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001145168.1 | 100% | 100% | |
| 2 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001145169.1 | 91.6% | 91.1% | (many diffs) |
| 3 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_011532622.2 | 90.3% | 89.3% | (many diffs) |
| 4 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001321971.2 | 90.2% | 87.5% | (many diffs) |
| 5 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_011532621.3 | 90% | 87% | (many diffs) |
| 6 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_017003505.1 | 89% | 86.2% | (many diffs) |
| 7 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_011532623.2 | 88.8% | 87.3% | (many diffs) |
| 8 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_153835.4 | 78.2% | 75.5% | (many diffs) |
| 9 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | XM_017003506.2 | 75.1% | 74.4% | (many diffs) |
| 10 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NR_135916.2 | 62.2% | (many diffs) | |
| 11 | human | 165082 | ADGRF3 | adhesion G protein-coupled ... | NM_001321975.2 | 61.2% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3312
- ORF length:
- 3237
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggtc tgttcggctg ccccactgct gctcctggcc acaactcttc 121 ccctgctggg gtcaccagtt gcccaagcat cccaacctgt aagtgagact ggggtgagac 181 ccagggaagg tctgcagagg cgacaatggg gacccctgat tgggagagac aaagcatgga 241 atgaaaggat agacagaccc ttccctgcct gccccatccc cctatcttct agctttggcc 301 gatggcccaa gggccagaca atgtgggccc agacctccac cctcaccctg acagaggagg 361 agttgggaca gagtcaggct ggaggggaat ctggatctgg gcagctcctg gaccaagaga 421 atggagcagg ggaatcagcg ctggtctccg tctatgtaca tctggacttt ccagataaga 481 cctggccccc tgaactctcc aggacactga ctctccctgc tgcctcagct tcctcttccc 541 caaggcctct tctcactggc ctcagactca caacagagtg taatgtcaac cacaagggga 601 atttctattg tgcttgcctc tctggctacc agtggaacac cagcatctgc ctccattacc 661 ctccttgtca aagcctccac aaccaccagc cttgtggctg ccttgtcttc agccatcccg 721 aacccgggta ctgccagttg ctgccacctg ggtcccctgt cacctgcctc cctgcagtcc 781 ccgggatcct caacctgaac tcccagctgc agatgcctgg tgacacgctg agcctgactc 841 tccatctgag ccaggaggcc accaacctga gctggttcct gaggcaccca gggagcccca 901 gtcccatcct cctgcagcca gggacacagg tgtctgtgac ttccagccac ggccaggctg 961 ccctcagcgt ctccaacatg tcccatcact gggcaggtga gtacatgagc tgcttcgagg 1021 cccagggctt caagtggaac ctgtatgagg tggtgagggt gcccttgaag gcgacagatg 1081 tggctcgact tccataccag ctgtccatct cctgtgccac ctcccctggc ttccagctga 1141 gctgctgcat ccccagcaca aacctggcct acaccgcggc ctggagccct ggagagggca 1201 gcaaagcttc ctccttcaac gagtcaggct ctcagtgctt tgtgctggct gttcagcgct 1261 gcccgatggc tgacaccacg tacgcttgtg acctgcagag cctgggcctg gctccactca 1321 gggtccccat ctccatcacc atcatccagg atggagacat cacctgccct gaggacgcct 1381 cggtgctcac ctggaatgtc accaaggctg gccacgtggc acaggcccca tgtcctgaga 1441 gcaagagggg catagtgagg aggctctgtg gggctgacgg agtctggggg ccggtccaca 1501 gcagctgcac agatgcgagg ctcctggcct tgttcactag aaccaagctg ctgcaggcag 1561 gccagggcag tcctgctgag gaggtgccac agatcctggc acagctgcca gggcaggcgg 1621 cagaggcaag ttcaccctcc gacttactga ccctgctgag caccatgaaa tacgtggcca 1681 aggtggtggc agaggccaga atacagcttg accgcagagc cctgaagaat ctcctgattg 1741 ccacagacaa ggtcctagat atggacacca ggtctctgtg gaccctggcc caagcccgga 1801 agccctgggc aggctcgact ctcctgctgg ctgtggagac cctggcatgc agcctgtgcc 1861 cacaggacca ccccttcgcc ttcagcttac ccaatgtgct gctgcagagc cagctgtttg 1921 gacccacgtt tcctgctgac tacagcatct ccttccctac tcggccccca ctgcaggctc 1981 agattcccag gcactcactg gccccattgg tccgtaatgg aactgaaata agtattacta 2041 gcctggtgct gcgaaaactg gaccaccttc tgccctcaaa ctatggacaa gggctggggg 2101 attccctcta tgccactcct ggcctggtcc ttgtcatttc catcatggca ggtgaccggg 2161 ccttcagcca gggagaggtc atcatggact ttgggaacac agatggttcc cctcactgtg 2221 tcttctggga tcacagtctc ttccagggca gggggggttg gtccaaagaa gggtgccagg 2281 cacaggtggc cagtgccagc cccactgctc agtgcctctg ccagcacctc actgccttct 2341 ccgtcctcat gtccccacac actgttccgg aagaacccgc tctggcgctg ctgactcaag 2401 tgggcttggg agcttccata ctggcgctgc ttgtgtgcct gggtgtgtac tggctggtgt 2461 ggagagtcgt ggtgcggaac aagatctcct atttccgcca cgccgccctg ctcaacatgg 2521 tgttctgctt gctggccgca gacacttgct tcctgggcgc cccattcctc tctccagggc 2581 cccgaagccc gctctgcctt gctgccgcct tcctctgtca tttcctctac ctggccacct 2641 ttttctggat gctggcgcag gccctggtgt tggcccacca gctgctcttt gtctttcacc 2701 agctggcaaa gcaccgagtt ctccccctca tggtgctcct gggctacctg tgcccactgg 2761 ggttggcagg tgtcaccctg gggctctacc tacctcaagg gcaatacctg agggaggggg 2821 aatgctggtt ggatgggaag ggaggggcgt tatacacctt cgtggggcca gtgctggcca 2881 tcataggcgt gaatgggctg gtactagcca tggccatgct gaagttgctg agaccttcgc 2941 tgtcagaggg acccccagca gagaagcgcc aagctcTGCT GGGGGTGATC AAAGCCCTGC 3001 TCATTCTTAC ACCCATCTTT GGCCTCACCT GGGGGCTGGG CCTGGCCACT CTGTTAGAGG 3061 AAGTCTCCAC GGTCCCTCAT TACATCTTCA CCATTCTCAA CACCCTCCAG GGCGTCTTCA 3121 TCCTATTGTT TGGTTGCCTC ATGGACAGGA AGATACAAGA AGCTTTGCGC AAACGCTTCT 3181 GCCGCGCCCA AGCCCCCAGC TCCACCATCT CCCTGGTGAG TTGCTGCCTT CAGATCCTCA 3241 GCTGTGCATC CAAGAGCATG TCAGAAGGCA TTCCATGGCC CTCCTCAGAG GACATGGGCA 3301 CAGCCAGAAG CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3361 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3421 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC ACCGCCCCCT TGCGGCTGGG 3481 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t