Construct: ORF TRCN0000492275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010353.1_s317c1
- Derived from:
- ccsbBroadEn_00405
- DNA Barcode:
- ATGACTTTTTTCAACCCAACAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYLC2 (1539)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1539 | CYLC2 | cylicin 2 | NM_001340.5 | 96.2% | 95.9% | 1002T>G;1004_1005delCAinsGC;1009_1044del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1077
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctctccca agattccaaa gagtaaactt tgggccatat gataattaca 121 ttccagtcag tgaattaagc aaaaaatcat ggaatcagca acactttgcc ctgttatttc 181 ccaaaccaca acggccagga accaaaagga gatcaaaacc ttctcaaata cgggacaaca 241 cggtttctat aattgatgaa gaacaattaa gaggagatcg tagacaacca ttatggatgt 301 accgttcttt aatgagaatt tctgagagac catctgttta tttagctgcc aggaggcagc 361 ctctcaaacc aactcgtact gtcgaggtgg attctaaagc agcagaaatt ggtaagaaag 421 gtgaagacaa gacaacacag aaggacacaa cagattcgga atcagaatta aaacaaggaa 481 aaaaagattc aaagaaaggc aaggatatag agaaaggaaa agaagaaaag ctagatgcaa 541 agaaagatag caaaaaaggt aaaaaggatg cagagaaggg caaagactca gcaacagaat 601 ctgaagatga aaaaggaggt gcaaagaaag ataacaaaaa agataaaaag gattcaaaca 661 aaggcaaaga ctcggcaaca gaatctgaag gtGAAAAAGG AGGTACAGAG AAAGATAGCA 721 AAAAAGGTAA AAAGGATTCA AAGAAGGGCA AGGATTCAGC CATAGAATTA CAAGCTGTAA 781 AAGCAGATGA AAAGAAGGAT GAGGATGGAA AAAAAGATGC AAACAAAGGT GATGAATCGA 841 AGGATGCCAA GAAAGATGCA AAGGAGATTA AAAAAGGTAA GAAAGATAAG AAGAAGCCCA 901 GTAGTACAGA CAGTGACTCA AAGGATGATG TCAAGAAAGA GTCTAAGAAG GACGCCACGA 961 AAGATGCCAA GAAAGTTGCC AAGAAAGATA CTGAGAAAGA ATCTGCTGAT TCAAAGAAGG 1021 ATGCAAAGAA AAATGCTAAG AAGGATGCAA AGAAGGATGC AAAGAAGAAG GGCAAGTTGC 1081 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1201 ATATATCTTG TGGAAAGGAC GAATGACTTT TTTCAACCCA ACAAACACGC GTTAAGTCga 1261 caatcaacct ctggattaca aaatttgtga aagatt