Construct: ORF TRCN0000492288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016668.2_s317c1
- Derived from:
- ccsbBroadEn_14346
- DNA Barcode:
- TTAACGGCAGGAAGTGCCAACGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D31 (93594)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363156.1 | 60% | 60% | 1_483del;822T>C;1136_1330del |
| 2 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363155.1 | 37.9% | 37.9% | 1_1668del;2007T>C |
| 3 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001330606.2 | 35.3% | 35.3% | 1_1668del;2007T>C;2321_2515del |
| 4 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XM_017013984.1 | 35.3% | 35.3% | 1_1668del;2007T>C;2321_2515del |
| 5 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363153.1 | 34.6% | 33% | (many diffs) |
| 6 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363154.1 | 34.4% | 34.4% | 1_1668del;2007T>C;2088_2089ins93 |
| 7 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363148.1 | 33.9% | 33.9% | 1_1983del;2322T>C |
| 8 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XM_005251104.1 | 32.4% | 30.8% | (many diffs) |
| 9 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363149.1 | 32.1% | 32.1% | 1_1953del;2292T>C;2606_2800del |
| 10 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363152.1 | 32.1% | 32.1% | (many diffs) |
| 11 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_145647.4 | 31.8% | 31.8% | 1_1983del;2322T>C;2636_2830del |
| 12 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001145088.2 | 30.8% | 30.8% | 1_1983del;2322T>C;2403_2404ins93 |
| 13 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XR_002956653.1 | 30.7% | (many diffs) | |
| 14 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363151.2 | 29.3% | 27.3% | (many diffs) |
| 15 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XR_928364.1 | 28.9% | (many diffs) | |
| 16 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | NM_001363150.1 | 28.9% | 28.9% | (many diffs) |
| 17 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XM_011517379.2 | 27.6% | 25.6% | (many diffs) |
| 18 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XR_001745625.1 | 27.2% | (many diffs) | |
| 19 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XR_001745622.1 | 26.3% | (many diffs) | |
| 20 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XR_928365.2 | 26.1% | (many diffs) | |
| 21 | human | 93594 | TBC1D31 | TBC1 domain family member 31 | XR_001745623.1 | 19.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct taatgttttt gttgcactga caaaagggca gtatccagta tttaatcaat 121 atccaaagtt tattgtggac tatcaaacac aggaacgaga aagaataagg aatgatgaat 181 tggattactt aagagagagg cagacagttg aagatatgca agctaaagtc gaccagcaaa 241 gagttgaaga tgaagcttgg taccagaaac aggagctgct tcgtaaagct gaagaaacaa 301 gaagagaaat gctcttacaa gaggaggaga aaatgataca acaaagacag aggctagctg 361 ctgtgaaaag agagctgaaa gtaaaggaaa tgcacttaca agacgctgca agaaggcgtt 421 ttctgaagct tcagcaagat caacaggaaa tggaactaag aagactggat gatgaaattg 481 ggagaaaggt atatatgaga gatcgagaaa ttgctgccac agccagagac ctagaaatga 541 gacagctgga actcgaatca caaaagagac tttatgagaa gaatcttact gaaaatcaag 601 aagctcttGC AAAAGAAATG CGAGCAGATG CAGATGCCTA TAGACGAAAA GTGGATCTTG 661 AAGAACACAT GTTTCATAAG CTGATAGAAG CAGGTGAAAC CCAGAGCCAG AAAACTCAGA 721 AGTGGAAGGA AGCTGAAGGA AAAGAGTTCC GTTTGAGATC AGCAAAGAAA GCTTCTGCTC 781 TTTCAGATGC GTCTAGAAAG TGGTTTTTAA AGCAAGAGAT AAATGCGGCT GTAGAACATG 841 CTGAAAATCC ATGTCATAAA GAAGAACCCA GGTTCCAAAA TGAACAGGAC TCAAGCTGTT 901 TGCCTAGAAC CTCACAATTA AATGACTCTT CTGAAATGGA TCCCTCAACA CAGATTTCTT 961 TAAATAGAAG AGCAGTAGAA TGGGACACCA CGGGACAGAA TCTTATTAAG AAAGTGAGAA 1021 ATCTTCGCCA GAGACTCACT GCCCGGGCTC GTCACAGATG TCAAACCCCT CATCTTTTGG 1081 CTGCATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTAACGGCA GGAAGTGCCA ACGTAACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt