Construct: ORF TRCN0000492294
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007976.1_s317c1
- Derived from:
- ccsbBroadEn_06612
- DNA Barcode:
- GGTATACCCTACTGTGTTATCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYOC (4653)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492294
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4653 | MYOC | myocilin | NM_000261.2 | 99.9% | 99.8% | 7T>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gatcttctgt gcacgttgct gcagctttgg gcctgagatg ccagctgtcc 121 agctgctgct tctggcctgc ctggtgtggg atgtgggggc caggacagct cagctcagga 181 aggccaatga ccagagtggc cgatgccagt ataccttcag tgtggccagt cccaatgaat 241 ccagctgccc agagcagagc caggccatgt cagtcatcca taacttacag agagacagca 301 gcacccaacg cttagacctg gaggccacca aagctcgact cagctccctg gagagcctcc 361 tccaccaatt gaccttggac caggctgcca ggccccagga gacccaggag gggctgcaga 421 gggagctggg caccctgagg cgggagcggg accagctgga aacccaaacc agagagttgg 481 agactgccta cagcaacctc ctccgagaca agtcagttct ggaggaagag aagaagcgac 541 taaggcaaga aaatgagaat ctggccagga ggttggaaag cagcagccag gaggtagcaa 601 ggctgagaag gggccagtgt ccccagaccc gagacactgc tcgggctgtg ccaccaggct 661 ccagagaagt ttctacgtgg aatttggaca ctttggcctt ccaggaactg aagtccgagc 721 taactgaagt tcctgcttcc cgaattttga aggagagccc atctggctat ctcaggagtg 781 gagagggaga caccggatgt ggagaactag tttgggtagg agagcctctc acgctgagaa 841 cagcagaaac aattactggc aagtatggtg tgtggatgcg agaccccaag cccacctacc 901 cctacaccca ggagaccacg tggagaatcg acacagttgg cacggatgtc cgccaggttt 961 ttgagtatga cctcatcagc cagtttatgc agggctaccc ttctaaggtt cacatactgc 1021 ctaggccact ggaaagcacg ggtgctgtgg tgtactcggg gagcctctat ttccagggcg 1081 ctgagtccag aactgtcata agatATGAGC TGAATACCGA GACAGTGAAG GCTGAGAAGG 1141 AAATCCCTGG AGCTGGCTAC CACGGACAGT TCCCGTATTC TTGGGGTGGC TACACGGACA 1201 TTGACTTGGC TGTGGATGAA GCAGGCCTCT GGGTCATTTA CAGCACCGAT GAGGCCAAAG 1261 GTGCCATTGT CCTCTCCAAA CTGAACCCAG AGAATCTGGA ACTCGAACAA ACCTGGGAGA 1321 CAAACATCCG TAAGCAGTCA GTCGCCAATG CCTTCATCAT CTGTGGCACC TTGTACACCG 1381 TCAGCAGCTA CACCTCAGCA GATGCTACCG TCAACTTTGC TTATGACACA GGCACAGGTA 1441 TCAGCAAGAC CCTGACCATC CCATTCAAGA ACCGCTATAA GTACAGCAGC ATGATTGACT 1501 ACAACCCCCT GGAGAAGAAG CTCTTTGCCT GGGACAACTT GAACATGGTC ACTTATGACA 1561 TCAAGCTCTC CAAGATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGTATAC CCTACTGTGT 1741 TATCACCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt