Construct: ORF TRCN0000492340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021847.2_s317c1
- DNA Barcode:
- GACTCGCTTAACAACAAAGGAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TP53BP2 (7159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492340
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | XM_011544268.2 | 97.9% | 97.2% | 1_61del;87_88insGGTA |
| 2 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | NM_005426.2 | 95.8% | 95.8% | 0_1ins129 |
| 3 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | XM_011544269.2 | 95.8% | 95.8% | 0_1ins129 |
| 4 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | NM_001031685.3 | 92.1% | 91.5% | 1_262del;288_289insGGTA |
| 5 | mouse | 209456 | Trp53bp2 | transformation related prot... | NM_173378.2 | 79.7% | 82.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3213
- ORF length:
- 3144
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaacgccccc ctggcaggga cattggtagt gagtggacca agatctcagg 121 atccaagttt aaaaagaaat ggtgtaaaag ttcctggtga atatcgaaga aaggagaacg 181 gtgttaatag tcctaggatg gatctgactc ttgctgaact tcaggaaatg gcatctcgcc 241 agcagcaaca gattgaagcc cagcaacaat tgctggcaac taaggaacag cgcttaaagt 301 ttttgaaaca acaagatcag cgacaacagc aacaagttgc tgagcaggag aaacttaaaa 361 ggctaaaaga aatagctgag aatcaggaag ctaagctaaa aaaagtgaga gcacttaaag 421 gccacgtgga acagaagaga ctaagcaatg ggaaacttgt ggaggaaatt gaacagatga 481 ataatttgtt ccagcaaaaa cagagggagc tcgtcctggc tgtgtcaaaa gtagaagaac 541 tgaccaggca gctagagatg ctcaagaacg gcaggatcga cagccaccat gacaatcagt 601 ctgcagtggc tgagcttgat cgcctctata aggagctgca gctaagaaac aaattgaatc 661 aagagcagaa tgccaagcta caacaacaga gggagtgttt gaataagcgt aattcagaag 721 tggcagtcat ggataagcgt gttaatgagc tgagggaccg gctgtggaag aagaaggcag 781 ctctacagca aaaagaaaat ctaccagttt catctgatgg aaatcttccc cagcaagccg 841 cgtcagcccc aagccgtgtg gctgcagtag gtccctatat ccagtcgtct actatgcctc 901 ggatgccctc aaggcctgaa ttgctggtga agccagccct gccggatggt tccttggtca 961 ttcaggcttc agaggggccg atgaaaatac agacactgcc caacatgaga tctggggctg 1021 cttcacaaac taaaggctct aaaatccatc cagttggccc tgattggagt ccttcaaatg 1081 cagatctttt cccaagccaa ggctctgctt ctgtacctca aagcactggg aatgctctgg 1141 atcaagttga tgatggagag gttccgctga gggagaaaga gaagaaagtg cgtccgttct 1201 caatgtttga tgcagtagac cagtccaatg ccccaccttc ctttggtact ctgaggaaga 1261 accagagcag tgaagatatc ttgcgggatg ctcaggttgc aaataaaaat gtggctaaag 1321 taccacctcc tgttcctaca aaaccaaaac agattaattt gccttatttt ggacaaacta 1381 atcagccacc ttcagacatt aagccagacg gaagttctca gcagttgtca acagttgttc 1441 cgtccatggg aactaaacca aaaccagcag ggcagcagcc gagagtgctg ctatctccca 1501 gcataccttc ggttggccaa gaccagaccc tttctccagg ttctaagcaa gaaagtccac 1561 ctgctgctgc cgtccggccc tttactcccc agccttccaa agacacctta cttccaccct 1621 tcagaaaacc ccagaccgtg gcagcaagtt caatatattc catgtatacg caacagcagg 1681 cgccaggaaa aaacttccag caggctgtgc agagcgcgtt gaccaagact cataccagag 1741 ggccacactt ttcaagtgta tatggtaagc ctgtaattgc tgctgcccag aatcaacagc 1801 agcacccaga gaacatttat tccaatagcc agggcaagcc tggcagtcca gaacctgaaa 1861 cagagcctgt ttcttcagtt caggagaacc atgaaaacga aagaattcct cggccactca 1921 gcccaactaa attactgcct ttcttatcta atccttaccg aaaccagagt gatgctgacc 1981 tagaagcctt acgaaagaaa ctgtctaacg caccaaggcc tctaaagaaa cgtagttcta 2041 ttacagagcc agagggtcct aatgggccaa atattcagaa gcttttatat cagaggacca 2101 ccatagcggc catggagacc atctctgtcc catcataccc atccaagtca gcttctgtga 2161 ctgccagctc agaaagccca gtagaaatcc agaatccata tttacatgtg gagcccgaaa 2221 aggaggtggt ctctctggtt cctgaatcat tgtccccaga ggatgtgggg aatgccagta 2281 cagagaacag tgacatgcca gctccttctc caggccttga ttatgagcct gagggagtcc 2341 cagacaacag cccaaatctc cagaataacc cagaagaacc aaatccagag gctccacatg 2401 tgcttgatgt gtacctggag gagtaccctc catacccacc cccaccatac ccatctgggg 2461 agcctgaagg gcccggagaa gactcggtga gcatgcgccc gcctgaaatc accgggcagg 2521 tctctctgcc tcctggtaaa aggacaaact tgcgtaaaac tggctcagag cgtatcgctc 2581 atggaatgag ggtgaaattc aacccccttg ctttactgct agattcgtct ttggagggag 2641 aatttgacct tgtacagaga attatttatg aggttgatga cccaagcctc cccaatgatg 2701 aaggcatcac ggctcttcac aatgctgtgt gtgcaggcca cacagaaatc gttaagttcc 2761 tggtacagtt tggtgtaaat gtaaatgctg ctgatagtga tggatggact ccattacatt 2821 gtgctgccTC ATGTAACAAC GTCCAAGTGT GTAAGTTTTT GGTGGAGTCA GGAGCCGCTG 2881 TGTTTGCCAT GACCTACAGT GACATGCAGA CTGCTGCAGA TAAGTGCGAG GAAATGGAGG 2941 AAGGCTACAC TCAGTGCTCC CAATTTCTTT ATGGAGTTCA GGAGAAGATG GGCATAATGA 3001 ATAAAGGAGT CATTTATGCG CTTTGGGATT ATGAACCTCA GAATGATGAT GAGCTGCCCA 3061 TGAAAGAAGG AGACTGCATG ACAATCATCC ACAGGGAAGA CGAAGATGAA ATCGAATGGT 3121 GGTGGGCGCG CCTTAATGAT AAGGAGGGAT ATGTTCCACG TAACTTGCTG GGACTGTACC 3181 CAAGAATTAA ACCAAGACAA AGGAGCTTGG CCTGAGACCC AGCTTTCTTG TACAAAGTGG 3241 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3301 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3361 AGACTCGCTT AACAACAAAG GAATCACGCG TTAAGTCgac aatcaacctc tggattacaa 3421 aatttgtgaa agatt