Construct: ORF TRCN0000492352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004469.1_s317c1
- Derived from:
- ccsbBroadEn_13580
- DNA Barcode:
- GTAGGAGCAACTAACCATCTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD42 (338699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_001300977.2 | 100% | 100% | |
2 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_001300976.2 | 85.7% | 84.7% | 587_595delGTGGCAGCCinsA;597T>C;603_690del |
3 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_182603.4 | 40.6% | 40% | (many diffs) |
4 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_001300972.2 | 39.3% | 38.8% | (many diffs) |
5 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_001300973.2 | 37.9% | 37.4% | (many diffs) |
6 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_001300975.2 | 37.5% | 37% | (many diffs) |
7 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NR_125355.2 | 16.8% | (many diffs) | |
8 | mouse | 73845 | Ankrd42 | ankyrin repeat domain 42 | XM_017312337.1 | 84.1% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 663
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccggggtg gccaattcag gcccctccac ttcctctagg gagactgcaa 121 acccctgttc caggaagaag gtgcattttg gcagcataca tgatgcagta cgagctggag 181 atgtaaagca gctttcagaa atagtggtac gtggagccag cattaatgaa cttgatgttc 241 tccataagtt taccccttta cattgggcag cacattctgg aagtttggag tgtcttcatt 301 ggctgctctg gcatggagct gatatcacac acgtaacaac gagaggttgg acagcatctc 361 acatagctgc aatcaggggt caggatgctt GTGTACAGGC TCTTATAATG AATGGAGCAA 421 ATCTGACAGC CCAGGATGAC CGGGGATGCA CTCCTTTACA TCTTGCTGCA ACTCATGGAC 481 ATTCTTTCAC TTTACAAATA ATGCTCCGAA GTGGAGTGGA TCCCAGTGTG ACTGATAAGA 541 GAGAATGGAG ACCTGTGCAT TATGCAGCTT TTCATGGGCG GCTTGGCTGC TTGCAACTTC 601 TTGTTAAATG GGGTTGTAGC ATAGAAGATG TGGACTACAA TGGAAACCTT CCAGAACCTC 661 CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAGT AGGAGCAACT AACCATCTCG GGACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt