Construct: ORF TRCN0000492361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019798.2_s317c1
- DNA Barcode:
- TGAAGAGACCCTCGCTAGATCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DRD2 (1813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1813 | DRD2 | dopamine receptor D2 | NM_016574.3 | 99.9% | 99.7% | 1242_1243insG |
| 2 | human | 1813 | DRD2 | dopamine receptor D2 | NM_000795.4 | 93.3% | 93.2% | 723_809del;1329_1330insG |
| 3 | human | 1813 | DRD2 | dopamine receptor D2 | XM_017017296.2 | 93.3% | 93.2% | 723_809del;1329_1330insG |
| 4 | mouse | 13489 | Drd2 | dopamine receptor D2 | XM_006509996.3 | 90.1% | 95.1% | (many diffs) |
| 5 | mouse | 13489 | Drd2 | dopamine receptor D2 | NM_010077.2 | 84.2% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1314
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggatccactg aatctgtcct ggtatgatga tgatctggag aggcagaact 121 ggagccggcc cttcaacggg tcagacggga aggcggacag accccactac aactactatg 181 ccacactgct caccctgctc atcgctgtca tcgtcttcgg caacgtgctg gtgtgcatgg 241 ctgtgtcccg cgagaaggcg ctgcagacca ccaccaacta cctgatcgtc agcctcgcag 301 tggccgacct cctcgtcgcc acactggtca tgccctgggt tgtctacctg gaggtggtag 361 gtgagtggaa attcagcagg attcactgtg acatcttcgt cactctggac gtcatgatgt 421 gcacggcgag catcctgaac ttgtgtgcca tcagcatcga caggtacaca gctgtggcca 481 tgcccatgct gtacaatacg cgctacagct ccaagcgccg ggtcaccgtc atgatctcca 541 tcgtctgggt cctgtccttc accatctcct gcccactcct cttcggactc aataacgcag 601 accagaacga gtgcatcatt gccaacccgg ccttcgtggt ctactcctcc atcgtctcct 661 tctacgtgcc cttcattgtc accctgctgg tctacatcaa gatctacatt gtcctccgca 721 gacgccgcaa gcgagtcaac accaaacgca gcagccgagc tttcagggcc cacctgaggg 781 ctccactaaa ggaggctgcc cggcgagccc aggagctgga gatggagatg ctctccagca 841 ccagcCCACC CGAGAGGACC CGGTACAGCC CCATCCCACC CAGCCACCAC CAGCTGACTC 901 TCCCCGACCC GTCCCACCAT GGTCTCCACA GCACTCCCGA CAGCCCCGCC AAACCAGAGA 961 AGAATGGGCA TGCCAAAGAC CACCCCAAGA TTGCCAAGAT CTTTGAGATC CAGACCATGC 1021 CCAATGGCAA AACCCGGACC TCCCTCAAGA CCATGAGCCG TAGGAAGCTC TCCCAGCAGA 1081 AGGAGAAGAA AGCCACTCAG ATGCTCGCCA TTGTTCTCGG CGTGTTCATC ATCTGCTGGC 1141 TGCCCTTCTT CATCACACAC ATCCTGAACA TACACTGTGA CTGCAACATC CCGCCTGTCC 1201 TGTACAGCGC CTTCACGTGG CTGGGCTATG TCAACAGCGC CGTGAACCCC ATCATCTACA 1261 CCACCTTCAA CATTGAGTTC CGCAAGGCCT TCCTGAAGAT CCTCCACTGC GACCCAGCTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1441 CTTGTGGAAA GGACGATGAA GAGACCCTCG CTAGATCGCA ACGCGTTAAG TCgacaatca 1501 acctctggat tacaaaattt gtgaaagatt