Construct: ORF ccsbBroad304_00010

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011294.1_s304c1, BRDN0000462201
Derived from:
ccsbBroadEn_00010
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACO1 (48)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00010
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 48 ACO1 aconitase 1 NM_001278352.1 100% 100%
2 human 48 ACO1 aconitase 1 NM_001362840.2 100% 100%
3 human 48 ACO1 aconitase 1 NM_002197.3 100% 100%
4 human 48 ACO1 aconitase 1 XM_011517888.2 100% 100%
5 mouse 11428 Aco1 aconitase 1 NM_007386.2 86.9% 93.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2733
ORF length:
2667
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag caacccattc gcacaccttg ctgagccatt ggatcctgta caaccaggaa
121agaaattctt caatttgaat aaattggagg attcaagata tgggcgctta ccattttcga
181tcagagttct tctggaagca gccattcgga attgtgatga gtttttggtg aagaaacagg
241atattgaaaa tattctacat tggaatgtca cgcagcacaa gaacatagaa gtgccattta
301agcctgctcg tgtcatcctg caggacttta cgggtgtgcc cgctgtggtt gactttgctg
361caatgcgtga tgctgtgaaa aagttaggag gagatccaga gaaaataaac cctgtctgcc
421ctgctgatct tgtaatagat cattccatcc aggttgattt caacagaagg gcagacagtt
481tacagaagaa tcaagacctg gaatttgaaa gaaatagaga gcgatttgaa tttttaaagt
541ggggttccca ggcttttcac aacatgcgga ttattccccc tggctcagga atcatccacc
601aggtgaattt ggaatatttg gcaagagtgg tatttgatca ggatggatat tattacccag
661acagcctcgt gggcacagac tcgcacacta ccatgattga tggcttgggc attcttggtt
721ggggtgtcgg tggtattgaa gcagaagctg tcatgctggg tcagccaatc agtatggtgc
781ttcctcaggt gattggctac aggctgatgg ggaagcccca ccctctggta acatccactg
841acatcgtgct caccattacc aagcacctcc gccaggttgg ggtagtgggc aaatttgtcg
901agttcttcgg gcctggagta gcccagttgt ccattgctga ccgagctacg attgctaaca
961tgtgtccaga gtacggagca actgctgcct ttttcccagt tgatgaagtt agtatcacgt
1021acctggtgca aacaggtcgt gatgaagaaa aattaaagta tattaaaaaa tatcttcagg
1081ctgtaggaat gtttcgagat ttcaatgacc cttctcaaga cccagacttc acccaggttg
1141tggaattaga tttgaaaaca gtagtgcctt gctgtagtgg acccaaaagg cctcaggaca
1201aagttgctgt gtccgacatg aaaaaggact ttgagagctg ccttggagcc aagcaaggat
1261ttaaaggatt ccaagttgct cctgaacatc ataatgacca taagaccttt atctatgata
1321acactgaatt cacccttgct catggttctg tggtcattgc tgccattact agctgcacaa
1381acaccagtaa tccgtctgtg atgttagggg caggattgtt agcaaagaaa gctgtggatg
1441ctggcctgaa cgtgatgcct tacatcaaaa ctagcctgtc tcctgggagt ggcgtggtca
1501cctactacct acaagaaagc ggagtcatgc cttatctgtc tcagcttggg tttgacgtgg
1561tgggctatgg ctgcatgacc tgcattggca acagtgggcc tttacctgaa cctgtggtag
1621aagccatcac acagggagac cttgtagctg ttggagtact atctggaaac aggaattttg
1681aaggtcgagt tcaccccaac acccgggcca actatttagc ctctcccccc ttagtaatag
1741catatgcaat tgctggaacc atcagaatcg actttgagaa agagccattg ggagtaaatg
1801caaagggaca gcaggtattt ctgaaagata tctggccgac tagagacgag atccaggcag
1861tggagcgtca gtatgtcatc ccggggatgt ttaaggaagt ctatcagaaa atagagactg
1921tgaatgaaag ctggaatgcc ttagcaaccc catcagataa gctgtttttc tggaattcca
1981aatctacgta tatcaaatca ccaccattct ttgaaaacct gactttggat cttcagcccc
2041ctaaatctat agtggatgcc tatgtgctgc taaatttggg agattcggta acaactgacc
2101acatctcccc agctggaaat attgcaagaa acagtcctgc tgctcgctac ttaactaaca
2161gaggcctaac tccacgagaa ttcaactcct atggctcccg ccgaggtaat gacgccgtca
2221tggcacgggg aacatttgcc aacattcgct tgttaaacag atttttgaac aagcaggcac
2281cacagactat ccatctgcct tctggggaaa tccttgatgt gtttgatgct gctgagcggt
2341accagcaggc aggccttccc ctgatcgttc tggctggcaa agagtacggt gcaggcagct
2401cccgagactg ggcagctaag ggccctttcc tgctgggaat caaagccgtc ctggccgaga
2461gctacgagcg cattcaccgc agtaacctgg ttgggatggg tgtgatccca cttgaatatc
2521tccctggtga gaatgcagat gccctggggc tcacagggca agaacgatac actatcatta
2581ttccagaaaa cctcaaacca caaatgaaag tccaggtcaa gctggatact ggcaagacct
2641tccaggctgt catgaggttt gacactgatg tggagctcac ttatttcctc aacgggggca
2701tcctcaacta catgatccgc aagatggcca agtacccaac tttcttgtac aaagtggttg
2761gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2821accggtggcg cgttaagtcg acaatcaacc tctggatta

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