Transcript: Human XM_011517888.2

PREDICTED: Homo sapiens aconitase 1 (ACO1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACO1 (48)
Length:
3467
CDS:
73..2742

Additional Resources:

NCBI RefSeq record:
XM_011517888.2
NBCI Gene record:
ACO1 (48)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011517888.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000056556 CCTACAAGAAAGCGGAGTCAT pLKO.1 1515 CDS 100% 4.950 6.930 N ACO1 n/a
2 TRCN0000056555 CGTATATCAAATCACCACCAT pLKO.1 1994 CDS 100% 2.640 3.696 N ACO1 n/a
3 TRCN0000056554 GCAGGATTGTTAGCAAAGAAA pLKO.1 1417 CDS 100% 5.625 4.500 N ACO1 n/a
4 TRCN0000333252 GCAGGATTGTTAGCAAAGAAA pLKO_005 1417 CDS 100% 5.625 4.500 N ACO1 n/a
5 TRCN0000344789 CCTTCCTCTTGAGGGTCATTT pLKO_005 3144 3UTR 100% 13.200 9.240 N ACO1 n/a
6 TRCN0000056557 CCTGCTGATCTTGTAATAGAT pLKO.1 427 CDS 100% 5.625 3.938 N ACO1 n/a
7 TRCN0000333172 CCTGCTGATCTTGTAATAGAT pLKO_005 427 CDS 100% 5.625 3.938 N ACO1 n/a
8 TRCN0000056553 CCAGGAAAGAAATTCTTCAAT pLKO.1 121 CDS 100% 5.625 3.375 N ACO1 n/a
9 TRCN0000333171 CCAGGAAAGAAATTCTTCAAT pLKO_005 121 CDS 100% 5.625 3.375 N ACO1 n/a
10 TRCN0000114452 GCAGACAGTTTACAGAAGAAT pLKO.1 478 CDS 100% 5.625 3.375 N Aco1 n/a
11 TRCN0000351217 GCAGACAGTTTACAGAAGAAT pLKO_005 478 CDS 100% 5.625 3.375 N Aco1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011517888.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00010 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00010 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000476593 GGTCCCAGGTGTCCTGTTAGATTG pLX_317 14.4% 100% 100% V5 n/a
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