Construct: ORF ccsbBroad304_00017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008777.1_s304c1, BRDN0000410842
- Derived from:
- ccsbBroadEn_00017
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTN2 (88)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00017
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 88 | ACTN2 | actinin alpha 2 | NM_001103.3 | 100% | 100% | |
| 2 | human | 88 | ACTN2 | actinin alpha 2 | NM_001278343.2 | 99% | 99.2% | (many diffs) |
| 3 | human | 88 | ACTN2 | actinin alpha 2 | NM_001278344.1 | 69.7% | 69.7% | 0_1ins735;48_158del |
| 4 | mouse | 11472 | Actn2 | actinin alpha 2 | NM_033268.4 | 91% | 99.2% | (many diffs) |
| 5 | mouse | 11472 | Actn2 | actinin alpha 2 | XM_011244263.2 | 64.7% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2748
- ORF length:
- 2682
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccagatagag cccggcgtgc agtacaacta cgtgtacgac gaggatgagt 121 acatgatcca ggaggaggag tgggaccgcg acctgctcct ggacccagcc tgggagaagc 181 agcagaggaa gaccttcact gcctggtgta actcccacct aaggaaagcc ggcacccaga 241 ttgagaacat cgaggaagac ttcaggaatg gccttaagct catgctgctt ttggaagtca 301 tctcagggga aaggctgccc aaacctgacc ggggaaaaat gcggttccac aaaattgcta 361 atgtcaacaa agctttggat tacatagcca gcaaaggggt gaaactggtg tccatcggcg 421 ctgaagaaat tgttgatggc aatgtgaaaa tgaccctggg tatgatctgg accatcatcc 481 ttcgctttgc tattcaggat atttcggttg aagaaacatc tgccaaagaa ggtctgctgc 541 tttggtgtca gaggaaaact gctccttata gaaatgtgaa cattcagaac ttccatacta 601 gctggaaaga tggccttgga ctctgtgccc tcatccaccg acaccggcct gacctcattg 661 actactcaaa gcttaacaag gatgacccca taggaaatat taacctggcc atggaaatcg 721 ctgagaagca cctggatatt cctaaaatgt tggatgctga agacatcgtg aacaccccta 781 aacccgatga aagagccatc atgacgtacg tctcttgctt ctaccacgct tttgcgggcg 841 cggagcaggc cgagacagcg gctaacagga tatgtaaggt tcttgctgtg aatcaagaga 901 atgagaggct gatggaagaa tatgagaggc tagcgagtga gcttttggaa tggattcgtc 961 gcacgatccc ctggctggag aaccggactc ccgagaagac catgcaagcc atgcagaaga 1021 agctggagga cttccgggat taccgccgga agcacaagcc acccaaggtg caggagaaat 1081 gccagctgga gatcaacttc aacacgctgc agaccaagct gcggatcagc aaccgtcctg 1141 ccttcatgcc ctccgagggc aagatggtgt cggatattgc tggtgcctgg cagaggctgg 1201 agcaggctga gaagggttac gaggagtggt tgctcaatga gattcggaga ctggagcgct 1261 tggaacacct ggctgagaag ttcaggcaga aggcctcaac gcacgagact tgggcttatg 1321 gcaaagagca gatcttgctg cagaaggatt acgagtcggc gtcgctgaca gaggtgcggg 1381 ctctgctgcg gaagcacgag gcgttcgaga gcgacctggc agcgcaccag gaccgcgtgg 1441 agcagatcgc agccatcgcg caggagctca atgaactgga ctatcacgac gctgtgaatg 1501 tcaatgatcg gtgccagaaa atttgtgacc agtgggaccg actgggaacg cttactcaga 1561 agaggagaga agccctagag agaatggaga aattgctaga aaccattgat cagcttcacc 1621 tggagtttgc caagagggct gctcctttca acaattggat ggagggcgct atggaggatc 1681 tgcaagatat gttcattgtc cacagcattg aggagatcca gagtctgatc actgcgcatg 1741 agcagttcaa ggccacgctg cccgaggcgg acggagagcg gcagtccatc atggccatcc 1801 agaacgaggt ggagaaggtg attcagagct acaacatcag aatcagctca agcaacccgt 1861 acagcactgt caccatggat gagctccgga ccaagtggga caaggtgaag caactcgtgc 1921 ccatccgcga tcaatccctg caggaggagc tggctcgcca gcatgctaac gagcgtctga 1981 ggcgccagtt tgctgcccaa gccaatgcca ttgggccctg gatccagaac aagatggagg 2041 agattgcccg gagctccatc cagatcacag gagccctgga agaccagatg aaccagctga 2101 agcagtatga gcacaacatc atcaactata agaacaacat cgacaagctg gagggagacc 2161 atcagctcat ccaggaggcc cttgtctttg acaacaagca cacgaactac acgatggagc 2221 acattcgtgt tggatgggag ctgctgctga caaccatcgc cagaaccatc aatgaggtgg 2281 agactcagat cctgacgaga gatgcgaagg gcatcaccca ggagcagatg aatgagttca 2341 gagcctcctt caaccacttt gacaggagga agaatggcct gatggatcat gaggatttca 2401 gagcctgcct gatttccatg ggttatgacc tgggtgaagc cgaatttgcc cgcattatga 2461 ccctggtaga tcccaacggg caaggcaccg tcaccttcca atccttcatc gacttcatga 2521 ctagagagac ggctgacacc gacactgccg agcaggtcat cgcctccttc cggatcctgg 2581 cttctgataa gccatacatc ctggcggagg agctgcgtcg ggagctgccc ccggatcagg 2641 cccagtactg catcaagagg atgcccgcct actcgggccc aggcagtgtg cctggtgcac 2701 tggattacgc tgcgttctct tccgcactct acggggagag cgatctgtgc ccaactttct 2761 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2821 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta