Construct: ORF ccsbBroad304_00047

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007322.1_s304c1, BRDN0000408592
Derived from:
ccsbBroadEn_00047
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
AKT2 (208)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00047
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 208 AKT2 AKT serine/threonine kinase 2 NM_001626.6 96.8% 58.7% (many diffs)
2 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526614.1 96.8% 58.7% (many diffs)
3 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526615.1 96.8% 58.7% (many diffs)
4 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526616.1 96.8% 58.7% (many diffs)
5 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526618.1 96.8% 58.7% (many diffs)
6 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526619.1 96.8% 58.7% (many diffs)
7 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526620.1 96.8% 58.7% (many diffs)
8 human 208 AKT2 AKT serine/threonine kinase 2 XM_017026470.2 96.8% 58.7% (many diffs)
9 human 208 AKT2 AKT serine/threonine kinase 2 XM_024451416.1 96.8% 58.7% (many diffs)
10 human 208 AKT2 AKT serine/threonine kinase 2 NM_001330511.1 88.8% 62.3% (many diffs)
11 human 208 AKT2 AKT serine/threonine kinase 2 XM_024451417.1 88.8% 62.3% (many diffs)
12 human 208 AKT2 AKT serine/threonine kinase 2 NM_001243027.3 84% 45.9% (many diffs)
13 human 208 AKT2 AKT serine/threonine kinase 2 NM_001243028.3 84% 45.9% (many diffs)
14 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526622.2 80.1% 70.5% (many diffs)
15 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_001110208.2 87.7% 57.5% (many diffs)
16 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_001331108.1 87.7% 57.5% (many diffs)
17 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_001331109.1 87.7% 57.5% (many diffs)
18 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_007434.4 87.7% 57.5% (many diffs)
19 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539477.1 87.7% 57.5% (many diffs)
20 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539480.1 87.7% 57.5% (many diffs)
21 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539481.3 87.7% 57.5% (many diffs)
22 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539482.1 79.7% 48.6% (many diffs)
23 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539483.1 79.7% 48.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1047
ORF length:
978
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaatgaggtg tctgtcatca aagaaggctg gctccacaag cgtggtgaat
121acatcaagac ctggaggcca cggtacttcc tgctgaagag cgacggctcc ttcattgggt
181acaaggagag gcccgaggcc cctgatcaga ctctaccccc cttaaacaac ttctccgtag
241cagaatgcca gctgatgaag accgagaggc cgcgacccaa cacctttgtc atacgctgcc
301tgcagtggac cacagtcatc gagaggacct tccacgtgga ttctccagac gagagggagg
361agtggatgcg ggccatccag atggtcgcca acagcctcaa gcagcgggcc ccaggcgagg
421accccatgga ctacaagtgt ggctccccca gtgactcctc cacgactgag gagatggaag
481tggcggtcag caaggcacgg gctaaagtga ccatgaatga cttcgactat ctcaaactcc
541ttggcaaggg aacctttggc aaagtcatcc tggtgcggga gaaggccact ggccgctact
601acgccatgaa gatcctgcgg aaggaagtca tcattgccaa ggatgaagtc gctcacacag
661tcaccgagag ccgggtcctc cagaacacca ggcacccgtt cctcactgcg ctgaagtatg
721ccttccagac ccacgaccgc ctgtgctttg tgatggagta tgccaacggg ggtgagctgt
781tcttccacct gtcccgggag cgtgtcttca cagaggagcg ggcccggttt tatggtgcag
841AGATCTTCTC GGCTCTCGAG TACTTGCACT CCCCGGGACG TGGTATACCG CGACATCAAG
901CTGGAAAACC TCATCCTGGA CAAAGACGGG CACGTCAAGA TCACTTGCCT TGGGTCTCTG
961CAAAGAGGGC ATCAGTGACG GGGCCCCCCA TGAAAACCTT CTTTGGGCCC CCCGAGTTCC
1021TGGCGCCCGA AGTTGCGGGA GGACAATGAC TTATGGCCGG GCCGTGGACT GGTGGGGGCT
1081GGGTGTGGTC ATGTCAGAGC AGATGTGCGG CCGCCTGCCC TTCTACAACC AGGGCCGCGA
1141GCGCCTCTCC GAGCTCATCC TCATGGAAGG AGATCCGCTT CCTGGCGCAC GCTCAGCCCG
1201AGGCCAAGTC CCTGCTTGCT GGGCTGCTTA AAGAACGACC CCAAGCAGAG GCTTGGTGGG
1261GGGCCCAGCG ATGCCAAGGA GGTCATGGAG CACAGGTTCT TCCTCAGCAT CAACTGGCAG
1321GACGTGGTCC AGAAGAAGCT CCTGCCCCCC TTCAAACCTC AGGTCACGTC CGAGGTCGAC
1381ACAAGGTACT TCGATGATGA ATTTACCGCC CAGTCCATCA CAATCAAACC CCCTGACCGC
1441TATGACAGCC TGGGCTTTCT GGAGCTGGAC CAGCGGACCC ACTTCCCCCA GTTCTCCCTC
1501TCGGCTCTCA TCCCCGAGTA CCCACTTTCT TGGACAAAAG GGTTGGGTAG CCCGTCCCTA
1561CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAGCTAGC GCTACCCGGG GGCGCGTTAA
1621GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT
1681GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTGTATCAT GCTATCCTCC

Download FASTA (ORF) (Full)

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