Construct: ORF ccsbBroad304_00087
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002773.1_s304c1, BRDN0000399618
- Derived from:
- ccsbBroadEn_00087
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FAS (355)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00087
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1191 | CLU | clusterin | NM_001831.4 | 56.9% | 5.3% | (many diffs) |
| 2 | human | 355 | FAS | Fas cell surface death rece... | NM_152871.4 | 56.4% | 29.9% | (many diffs) |
| 3 | human | 355 | FAS | Fas cell surface death rece... | NM_000043.6 | 56.2% | 28% | (many diffs) |
| 4 | human | 355 | FAS | Fas cell surface death rece... | NM_001320619.2 | 52.1% | 47.7% | (many diffs) |
| 5 | human | 1191 | CLU | clusterin | XM_006716284.3 | 51.2% | 4.8% | (many diffs) |
| 6 | human | 355 | FAS | Fas cell surface death rece... | NM_152872.4 | 51.2% | 42.7% | (many diffs) |
| 7 | human | 1191 | CLU | clusterin | NR_045494.1 | 27.3% | (many diffs) | |
| 8 | human | 1191 | CLU | clusterin | NR_038335.1 | 26% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 369
- ORF length:
- 303
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gggcatctgg accctcctac ctctggttct tacgtctgtt gctagattat 121 cgtccaaaag tgttaatgcc caagtgactg acatcaactc caagggattg gaattgagga 181 agactgttac tacagttgag actcagaact tggaaggcct gcatcatgat ggccaattct 241 gccataagcc ctgtcctcca ggtgaaagga aagctaggga ctgcacagtc aatggggatg 301 aaccagactg cgtgccctgc caagaaggga aggagtacac agacCCACGC CATGTTCCAG 361 CCCTTCCTTG AGATGATACA CGAGGCTCAG CAGGCCATGG ACATCCACTT CCATAGCCCG 421 GCCTTCCAGC ACCCGCCAAC AGAATTCATA CGAGAAGGCG ACGATGACCG GACTGTGTGC 481 CGGGAGATCC GCCACAACTC CACGGGCTGC CTGCGGATGA AGGACCAGTG TGACAAGTGC 541 CGGGAGATCT TGTCTGTGGA CTGTTCCACC AACAACCCCT CCCAGGCTAA GCTGCGGCGG 601 GAGCTCGACG AATCCCTCCA GGTCGCTGAG AGGTTGACCA GGAAATACAA CGAGCTGCTA 661 AAGTCCTACC AGTGGAAGAT GCTCAACACC TCCTCCTTGC TGGAGCAGCT GAACGAGCAG 721 TTTAACTGGG TGTCCCGGCT GGCAAACCTC ACGCAAGGCG AAGACCAGTA CTATCTGCGG 781 GTCACCACGG TGGCTTCCCA CACTTCTGAC TCGGACGTTC CTTCCGGTGT CACTGAGGTG 841 GTCGTGAAGC TCTTTGACTC TGATCCCATC ACTGTGACGG TCCCTGTAGA AGTCTCCAGG 901 AAGAACCCTA AATTTATGGA GACCGTGGCG GAGAAAGCGC TGCAGGAATA CCGCAAAAAG 961 CACCGGGAGG AGTGCCCAAC TTTCTTGTAC aaagtggttg gtaagcctat ccctaaccct 1021 ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg 1081 acaatcaacc tctggatta