Construct: ORF ccsbBroad304_00089
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005104.1_s304c1, BRDN0000406196
- Derived from:
- ccsbBroadEn_00089
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AQP3 (360)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00089
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 360 | AQP3 | aquaporin 3 (Gill blood group) | NM_004925.5 | 100% | 100% | |
| 2 | human | 360 | AQP3 | aquaporin 3 (Gill blood group) | NM_001318144.2 | 70.8% | 56.7% | 492_493ins218;659_711del |
| 3 | human | 360 | AQP3 | aquaporin 3 (Gill blood group) | XR_001746289.1 | 44.3% | 1_66del;439_580del;1085_1976del | |
| 4 | mouse | 11828 | Aqp3 | aquaporin 3 | NM_016689.2 | 88.3% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tcgacagaag gagctggtgt cccgctgcgg ggagatgctc cacatccgct 121 accggctgct ccgacaggcg ctggccgagt gcctggggac cctcatcctg gtgatgtttg 181 gctgtggctc cgtggcccag gttgtgctca gccggggcac ccacggtggt ttcctcacca 241 tcaacctggc ctttggcttt gctgtcactc tgggcatcct catcgctggc caggtctctg 301 gggcccacct gaaccctgcc gtgacctttg ccatgtgctt cctggctcgt gagccctgga 361 tcaagctgcc catctacacc ctggcacaga cgctgggagc cttcttgggt gctggaatag 421 tttttgggct gtattatgat gcaatctggc acttcgccga caaccagctt tttgtttcgg 481 gccccaatgg cacagccggc atctttgcta cctacccctc tggacacttg gatatgatca 541 atggcttctt tgaccagttc ataggcacag cctcccttat cgtgtgtgtg ctggccattg 601 ttgaccccta caacaacccc gtcccccgag gcctggaggc cttcaccgtg ggcctggtgg 661 tcctggtcat tggcacctcc atgggcttca actccggcta tgccgtcaac cctgcccggg 721 actttggccc ccgccttttt acagcccttg cgggctgggg ctctgcagtc ttcacgaccg 781 gccagcattg gtggtgggtg cccatcgtgt ccccactcct gggctccatt gcgggtgtct 841 tcgtgtacca gctgatgatc ggctgccacc tggagcagcc cccaccctcc aacgaggaag 901 agaatgtgaa gctggcccat gtgaagcaca aggagcagat ctgcccaact ttcttgtaca 961 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1021 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta