Construct: ORF ccsbBroad304_00115

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017521.1_s304c1, BRDN0000411110
Derived from:
ccsbBroadEn_00115
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ASPH (444)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00115
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 444 ASPH aspartate beta-hydroxylase NM_001164755.2 100% 100%
2 human 444 ASPH aspartate beta-hydroxylase NM_001164754.1 88.5% 80.1% (many diffs)
3 human 444 ASPH aspartate beta-hydroxylase XM_017013447.1 87.7% 87.4% (many diffs)
4 human 444 ASPH aspartate beta-hydroxylase NM_032466.4 86.2% 86.2% 490_618del
5 human 444 ASPH aspartate beta-hydroxylase NM_001164753.2 83.1% 82.8% (many diffs)
6 human 444 ASPH aspartate beta-hydroxylase XM_017013446.1 77.3% 69.3% (many diffs)
7 human 444 ASPH aspartate beta-hydroxylase XM_024447157.1 76.9% 76.8% 490_618del;936A>T;939_1050delinsT
8 human 444 ASPH aspartate beta-hydroxylase XM_017013445.1 75.7% 75.3% (many diffs)
9 human 444 ASPH aspartate beta-hydroxylase NM_001164752.2 73.2% 65.8% (many diffs)
10 human 444 ASPH aspartate beta-hydroxylase NM_032468.5 72.2% 71.9% (many diffs)
11 human 444 ASPH aspartate beta-hydroxylase NM_001164751.2 71.9% 71.6% (many diffs)
12 human 444 ASPH aspartate beta-hydroxylase XM_024447158.1 67.5% 67.1% (many diffs)
13 human 444 ASPH aspartate beta-hydroxylase NM_001164756.1 57.3% 42.1% (many diffs)
14 human 444 ASPH aspartate beta-hydroxylase XM_005251246.3 37.6% 37.6% 807A>T;810_2145delinsT
15 human 444 ASPH aspartate beta-hydroxylase XM_005251243.2 36.8% 36.8% 252_296del;852A>T;855_2190delinsT
16 human 444 ASPH aspartate beta-hydroxylase XM_017013438.1 36.7% 36.7% (many diffs)
17 human 444 ASPH aspartate beta-hydroxylase XM_017013437.2 36.2% 36.2% 807A>T;810_2226delinsT
18 human 444 ASPH aspartate beta-hydroxylase XM_017013430.1 35.5% 35.5% 252_296del;852A>T;855_2271delinsT
19 human 444 ASPH aspartate beta-hydroxylase NM_004318.4 35.5% 35.4% 490_618del;936A>T;939_2274delinsT
20 human 444 ASPH aspartate beta-hydroxylase XM_017013432.1 35.4% 35.4% (many diffs)
21 human 444 ASPH aspartate beta-hydroxylase XM_017013429.2 35.4% 35.3% (many diffs)
22 human 444 ASPH aspartate beta-hydroxylase XM_005251242.3 35.2% 32.9% (many diffs)
23 human 444 ASPH aspartate beta-hydroxylase XM_005251235.2 34.8% 34.7% (many diffs)
24 human 444 ASPH aspartate beta-hydroxylase XM_005251236.2 34.7% 34.6% (many diffs)
25 human 444 ASPH aspartate beta-hydroxylase XM_005251239.2 34.5% 32.3% (many diffs)
26 human 444 ASPH aspartate beta-hydroxylase XM_017013434.1 34.4% 32.2% (many diffs)
27 human 444 ASPH aspartate beta-hydroxylase XM_017013422.2 34.3% 34.2% 490_618del;936A>T;939_2355delinsT
28 human 444 ASPH aspartate beta-hydroxylase XM_005251247.2 34.2% 34.2% (many diffs)
29 human 444 ASPH aspartate beta-hydroxylase XM_017013424.2 34.1% 34.1% (many diffs)
30 human 444 ASPH aspartate beta-hydroxylase XM_017013428.2 33.9% 31.8% (many diffs)
31 human 444 ASPH aspartate beta-hydroxylase XM_017013419.1 33.6% 33.6% (many diffs)
32 human 444 ASPH aspartate beta-hydroxylase XM_017013420.1 33.5% 33.5% (many diffs)
33 human 444 ASPH aspartate beta-hydroxylase XM_017013425.1 33.3% 31.2% (many diffs)
34 human 444 ASPH aspartate beta-hydroxylase XM_017013444.1 33.1% 32.8% (many diffs)
35 human 444 ASPH aspartate beta-hydroxylase XM_024447155.1 33% 33% (many diffs)
36 human 444 ASPH aspartate beta-hydroxylase XM_017013421.1 31.9% 31.8% (many diffs)
37 human 444 ASPH aspartate beta-hydroxylase XM_017013440.1 31.2% 31% (many diffs)
38 human 444 ASPH aspartate beta-hydroxylase NM_001164750.2 31.2% 31% (many diffs)
39 human 444 ASPH aspartate beta-hydroxylase XM_017013439.1 31.1% 30.8% (many diffs)
40 human 444 ASPH aspartate beta-hydroxylase XM_005251240.1 30.6% 30.4% (many diffs)
41 human 444 ASPH aspartate beta-hydroxylase XM_017013431.1 30.1% 29.9% (many diffs)
42 human 444 ASPH aspartate beta-hydroxylase XM_005251244.1 30.1% 27.8% (many diffs)
43 human 444 ASPH aspartate beta-hydroxylase XM_017013433.1 30% 29.8% (many diffs)
44 human 444 ASPH aspartate beta-hydroxylase XM_024447156.1 29.7% 27.5% (many diffs)
45 human 444 ASPH aspartate beta-hydroxylase XM_017013426.1 29.6% 29.3% (many diffs)
46 human 444 ASPH aspartate beta-hydroxylase XM_017013427.1 29.4% 29.2% (many diffs)
47 human 444 ASPH aspartate beta-hydroxylase XM_017013435.1 29% 26.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
876
ORF length:
810
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccagcgtaag aatgccaaga gcagcggcaa cagcagcagc agcggctccg
121gcagcggtag cacgagtgcg ggcagcagca gccccggggc ccggagagag acaaagcatg
181gaggacacaa gaatgggagg aaaggcggac tctcaggaac ttcattcttc acgtggttta
241tggtgattgc attgctgggc gtctggacat ctgtagctgt cgtttggttt gatcttgttg
301actatgagga agttctagga aaactaggaa tctatgatgc tgatggtgat ggagattttg
361atgtggatga tgccaaagtt ttattaggac ttaaagagag atctacttca gagccagcag
421tcccgccaga agaggctgag ccacacactg agcccgagga gcaggttcct gtggaggcag
481aaccccagaa tatcgaagat gaagcaaaag aacaaattca gtcccttctc catgaaatgg
541tacacgcaga acatgaaacc gagcatagtt accacgtgga agagacagtt tcacaagact
601gtaatcagga tatggaagag atgatgtctg agcaggaaaa tccagattcc agtgaaccag
661tagtagaaga tgaaagattg caccatgata cagatgatgt aacataccaa gtctatgagg
721aacaagcagt atatgaacct ctagaaaatg aagggataga aatcacagaa gtaactgctc
781cccctgagga taatcctgta gaagattcac aggtaattgt agaagaagta agcatttttc
841ctgtggaaga acagcaggaa gtaccaccag atacttaccc aactttcttg tacaaagtgg
901ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
961ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.