Construct: ORF ccsbBroad304_00115

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017521.1_s304c1, BRDN0000411110
Derived from:
ccsbBroadEn_00115
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ASPH (444)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00115
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 444 ASPH aspartate beta-hydroxylase NM_001164755.2 100% 100%
2 human 444 ASPH aspartate beta-hydroxylase NM_001164754.1 88.5% 80.1% (many diffs)
3 human 444 ASPH aspartate beta-hydroxylase XM_017013447.1 87.7% 87.4% (many diffs)
4 human 444 ASPH aspartate beta-hydroxylase NM_032466.4 86.2% 86.2% 490_618del
5 human 444 ASPH aspartate beta-hydroxylase NM_001164753.2 83.1% 82.8% (many diffs)
6 human 444 ASPH aspartate beta-hydroxylase XM_017013446.1 77.3% 69.3% (many diffs)
7 human 444 ASPH aspartate beta-hydroxylase XM_024447157.1 76.9% 76.8% 490_618del;936A>T;939_1050delinsT
8 human 444 ASPH aspartate beta-hydroxylase XM_017013445.1 75.7% 75.3% (many diffs)
9 human 444 ASPH aspartate beta-hydroxylase NM_001164752.2 73.2% 65.8% (many diffs)
10 human 444 ASPH aspartate beta-hydroxylase NM_032468.5 72.2% 71.9% (many diffs)
11 human 444 ASPH aspartate beta-hydroxylase NM_001164751.2 71.9% 71.6% (many diffs)
12 human 444 ASPH aspartate beta-hydroxylase XM_024447158.1 67.5% 67.1% (many diffs)
13 human 444 ASPH aspartate beta-hydroxylase NM_001164756.1 57.3% 42.1% (many diffs)
14 human 444 ASPH aspartate beta-hydroxylase XM_005251246.3 37.6% 37.6% 807A>T;810_2145delinsT
15 human 444 ASPH aspartate beta-hydroxylase XM_005251243.2 36.8% 36.8% 252_296del;852A>T;855_2190delinsT
16 human 444 ASPH aspartate beta-hydroxylase XM_017013438.1 36.7% 36.7% (many diffs)
17 human 444 ASPH aspartate beta-hydroxylase XM_017013437.2 36.2% 36.2% 807A>T;810_2226delinsT
18 human 444 ASPH aspartate beta-hydroxylase XM_017013430.1 35.5% 35.5% 252_296del;852A>T;855_2271delinsT
19 human 444 ASPH aspartate beta-hydroxylase NM_004318.4 35.5% 35.4% 490_618del;936A>T;939_2274delinsT
20 human 444 ASPH aspartate beta-hydroxylase XM_017013432.1 35.4% 35.4% (many diffs)
21 human 444 ASPH aspartate beta-hydroxylase XM_017013429.2 35.4% 35.3% (many diffs)
22 human 444 ASPH aspartate beta-hydroxylase XM_005251242.3 35.2% 32.9% (many diffs)
23 human 444 ASPH aspartate beta-hydroxylase XM_005251235.2 34.8% 34.7% (many diffs)
24 human 444 ASPH aspartate beta-hydroxylase XM_005251236.2 34.7% 34.6% (many diffs)
25 human 444 ASPH aspartate beta-hydroxylase XM_005251239.2 34.5% 32.3% (many diffs)
26 human 444 ASPH aspartate beta-hydroxylase XM_017013434.1 34.4% 32.2% (many diffs)
27 human 444 ASPH aspartate beta-hydroxylase XM_017013422.2 34.3% 34.2% 490_618del;936A>T;939_2355delinsT
28 human 444 ASPH aspartate beta-hydroxylase XM_005251247.2 34.2% 34.2% (many diffs)
29 human 444 ASPH aspartate beta-hydroxylase XM_017013424.2 34.1% 34.1% (many diffs)
30 human 444 ASPH aspartate beta-hydroxylase XM_017013428.2 33.9% 31.8% (many diffs)
31 human 444 ASPH aspartate beta-hydroxylase XM_017013419.1 33.6% 33.6% (many diffs)
32 human 444 ASPH aspartate beta-hydroxylase XM_017013420.1 33.5% 33.5% (many diffs)
33 human 444 ASPH aspartate beta-hydroxylase XM_017013425.1 33.3% 31.2% (many diffs)
34 human 444 ASPH aspartate beta-hydroxylase XM_017013444.1 33.1% 32.8% (many diffs)
35 human 444 ASPH aspartate beta-hydroxylase XM_024447155.1 33% 33% (many diffs)
36 human 444 ASPH aspartate beta-hydroxylase XM_017013421.1 31.9% 31.8% (many diffs)
37 human 444 ASPH aspartate beta-hydroxylase XM_017013440.1 31.2% 31% (many diffs)
38 human 444 ASPH aspartate beta-hydroxylase NM_001164750.2 31.2% 31% (many diffs)
39 human 444 ASPH aspartate beta-hydroxylase XM_017013439.1 31.1% 30.8% (many diffs)
40 human 444 ASPH aspartate beta-hydroxylase XM_005251240.1 30.6% 30.4% (many diffs)
41 human 444 ASPH aspartate beta-hydroxylase XM_017013431.1 30.1% 29.9% (many diffs)
42 human 444 ASPH aspartate beta-hydroxylase XM_005251244.1 30.1% 27.8% (many diffs)
43 human 444 ASPH aspartate beta-hydroxylase XM_017013433.1 30% 29.8% (many diffs)
44 human 444 ASPH aspartate beta-hydroxylase XM_024447156.1 29.7% 27.5% (many diffs)
45 human 444 ASPH aspartate beta-hydroxylase XM_017013426.1 29.6% 29.3% (many diffs)
46 human 444 ASPH aspartate beta-hydroxylase XM_017013427.1 29.4% 29.2% (many diffs)
47 human 444 ASPH aspartate beta-hydroxylase XM_017013435.1 29% 26.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
876
ORF length:
810
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccagcgtaag aatgccaaga gcagcggcaa cagcagcagc agcggctccg
121gcagcggtag cacgagtgcg ggcagcagca gccccggggc ccggagagag acaaagcatg
181gaggacacaa gaatgggagg aaaggcggac tctcaggaac ttcattcttc acgtggttta
241tggtgattgc attgctgggc gtctggacat ctgtagctgt cgtttggttt gatcttgttg
301actatgagga agttctagga aaactaggaa tctatgatgc tgatggtgat ggagattttg
361atgtggatga tgccaaagtt ttattaggac ttaaagagag atctacttca gagccagcag
421tcccgccaga agaggctgag ccacacactg agcccgagga gcaggttcct gtggaggcag
481aaccccagaa tatcgaagat gaagcaaaag aacaaattca gtcccttctc catgaaatgg
541tacacgcaga acatgaaacc gagcatagtt accacgtgga agagacagtt tcacaagact
601gtaatcagga tatggaagag atgatgtctg agcaggaaaa tccagattcc agtgaaccag
661tagtagaaga tgaaagattg caccatgata cagatgatgt aacataccaa gtctatgagg
721aacaagcagt atatgaacct ctagaaaatg aagggataga aatcacagaa gtaactgctc
781cccctgagga taatcctgta gaagattcac aggtaattgt agaagaagta agcatttttc
841ctgtggaaga acagcaggaa gtaccaccag atacttaccc aactttcttg tacaaagtgg
901ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
961ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)