Construct: ORF ccsbBroad304_00137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015876.1_s304c1, BRDN0000405087
- Derived from:
- ccsbBroadEn_00137
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1E1 (529)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00137
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 529 | ATP6V1E1 | ATPase H+ transporting V1 s... | NM_001696.4 | 100% | 100% | |
| 2 | human | 529 | ATP6V1E1 | ATPase H+ transporting V1 s... | NM_001039366.1 | 90.2% | 90.2% | 31_32ins66 |
| 3 | human | 529 | ATP6V1E1 | ATPase H+ transporting V1 s... | NM_001039367.1 | 86.7% | 86.7% | 276_277ins90 |
| 4 | mouse | 11973 | Atp6v1e1 | ATPase, H+ transporting, ly... | NM_007510.2 | 91% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tcgccatggc tctcagcgat gctgacgtgc aaaagcagat aaagcatatg atggctttca 121 ttgaacaaga agccaatgag aaagcagaag aaatagatgc aaaggcagaa gaagagttca 181 acatagagaa aggtcggctt gtgcaaaccc aaagactaaa gattatggaa tattatgaga 241 agaaagagaa acagattgag cagcagaaga aaattcagat gtccaatttg atgaatcaag 301 cgagactcaa agtcctcaga gcaagagatg accttatcac agacctacta aatgaagcaa 361 aacagagact cagcaaggtg gtaaaagata caaccaggta ccaagtgctg ctggatggac 421 tggttctcca gggtttgtac cagttgctgg agccccgaat gattgttcgt tgcaggaaac 481 aagatttccc tctggtaaag gctgcagtgc agaaggcaat tcctatgtac aaaattgcca 541 ccaaaaacga tgttgatgtc caaattgacc aggagtccta cctgcctgaa gacatagctg 601 gtggagttga gatctataat ggagatcgta aaataaaggt ttccaacacc ctggaaagcc 661 ggctggatct catagcccag cagatgatgc cagaagtccg gggagccttg tttggtgcaa 721 atgccaacag gaagtttttg gactaancaa ctttcttgta caaagtggtt ggtaagccta 781 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 841 gcgttaagtc gacaatcaac ctctggatta