Transcript: Mouse NM_007510.2

Mus musculus ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Atp6v1e1 (11973)
Length:
1219
CDS:
66..746

Additional Resources:

NCBI RefSeq record:
NM_007510.2
NBCI Gene record:
Atp6v1e1 (11973)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007510.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000103775 GCTTCCTCTTTCTGTTCTAAT pLKO.1 806 3UTR 100% 13.200 9.240 N Atp6v1e1 n/a
2 TRCN0000302770 GCTTCCTCTTTCTGTTCTAAT pLKO_005 806 3UTR 100% 13.200 9.240 N Atp6v1e1 n/a
3 TRCN0000103777 CCTCATCACTGATCTGCTAAA pLKO.1 332 CDS 100% 10.800 7.560 N Atp6v1e1 n/a
4 TRCN0000302769 CCTCATCACTGATCTGCTAAA pLKO_005 332 CDS 100% 10.800 7.560 N Atp6v1e1 n/a
5 TRCN0000103779 CAAACGCAAAGACTGAAGATT pLKO.1 204 CDS 100% 5.625 3.938 N Atp6v1e1 n/a
6 TRCN0000103776 GCAAATGCCAATAGGAAGTTT pLKO.1 717 CDS 100% 5.625 3.938 N Atp6v1e1 n/a
7 TRCN0000302699 GCAAATGCCAATAGGAAGTTT pLKO_005 717 CDS 100% 5.625 3.938 N Atp6v1e1 n/a
8 TRCN0000103778 TGGAGCCTTGTTTGGTGCAAA pLKO.1 701 CDS 100% 4.950 3.465 N Atp6v1e1 n/a
9 TRCN0000302772 TGGAGCCTTGTTTGGTGCAAA pLKO_005 701 CDS 100% 4.950 3.465 N Atp6v1e1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007510.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00137 pDONR223 98.5% 91% 98.6% None (many diffs) n/a
2 ccsbBroad304_00137 pLX_304 0% 91% 98.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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