Construct: ORF ccsbBroad304_00177

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000046.1_s304c1, BRDN0000400282
Derived from:
ccsbBroadEn_00177
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BTD (686)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00177
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 686 BTD biotinidase NM_001281723.3 96.3% 96.3% 0_1ins60
2 human 686 BTD biotinidase NM_001281724.3 96.3% 96.3% 0_1ins60
3 human 686 BTD biotinidase NM_001281725.2 96.3% 96.3% 0_1ins60
4 human 686 BTD biotinidase NM_001323582.1 96.3% 96.3% 0_1ins60
5 human 686 BTD biotinidase NM_001370658.1 96.3% 96.3% 0_1ins60
6 human 686 BTD biotinidase XM_011534041.2 96.3% 96.3% 0_1ins60
7 human 686 BTD biotinidase XM_017007088.1 96.3% 96.3% 0_1ins60
8 human 686 BTD biotinidase XM_024453724.1 96.3% 96.3% 0_1ins60
9 human 686 BTD biotinidase NM_001370752.1 65.7% 62.6% (many diffs)
10 human 686 BTD biotinidase NM_001281726.1 31.1% 29.5% (many diffs)
11 human 686 BTD biotinidase NM_001370753.1 25.5% 24.8% (many diffs)
12 mouse 26363 Btd biotinidase XM_006519029.3 63.9% 65.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1695
ORF length:
1629
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gcatgcgcat attcagggcg gaaggcgcgc taagagcaga tttgtggtct
121gcattatgtc tggagccaga agtaagcttg ctcttttcct ctgcggctgt tacgtggttg
181ccctgggagc ccacaccggg gaggagagcg tggctgacca tcacgaggct gaatattatg
241tggctgccgt gtatgagcat ccatccatcc tgagtctgaa ccctctggct ctcatcagcc
301gccaagaggc cttggagctc atgaaccaga accttgacat ctatgaacag caagtgatga
361ctgcagccca aaaggatgta cagattatag tgtttccaga agatggcatt catggattca
421actttacaag aacatccatt tatccatttt tggacttcat gccgtctccc caggtggtca
481ggtggaaccc atgcctggag cctcaccgct tcaatgacac agaggtgctc cagcgcctga
541gttgtatggc catcagggga gatatgttct tggtggccaa tcttgggaca aaggagcctt
601gtcatagcag tgacccaagg tgcccaaaag atgggagata ccagttcaac acaaatgtcg
661tgttcagcaa taatggaacc cttgttgacc gctaccgtaa acacaacctc tactttgagg
721cagcattcga tgttcctctt aaagtggatc tcatcacctt tgataccccc tttgctggca
781ggtttggcat cttcacatgc tttgatatat tgttctttga ccctgccatc agagtcctca
841gagactacaa ggtgaagcat gttgtgtacc caactgcctg gatgaaccag ctcccactct
901tggcagcaat tgagattcag aaagcttttg ctgttgcctt tggcatcaac gttctggcag
961ctaatgtcca ccacccagtt ctggggatga caggaagtgg catacacacc cctctggagt
1021ccttttggta ccatgacatg gaaaatccca aaagtcacct tataattgcc caggtggcca
1081aaaatccagt gggtctcatt ggtgcagaga atgcaacagg tgaaacggac ccatcccata
1141gtaagttttt aaaaattttg tcaggcgatc cgtactgtga gaaggatgct caggaagtcc
1201actgtgatga ggccaccaag tggaacgtga atgctcctcc cacatttcac tctgagatga
1261tgtatgacaa tttcaccctg gtccctgtct ggggaaagga aggctatctc cacgtctgtt
1321ccaatggcct ctgctgttat ttactttacg agaggcccac cttatccaaa gagctgtatg
1381ccctgggggt ctttgatggg cttcacacag tacatggcac ttactacatc caagtgtgtg
1441ccctggtcag gtgtgggggt cttggcttcg acacctgtgg acaggaaatc acagaggcca
1501cggggatatt tgagtttcac ctgtggggca acttcagtac ttcctatatc tttcctttgt
1561ttctgacctc agggatgacc ctagaagtcc ctgaccagct tggctgggag aatgaccact
1621atttcctgag gaaaagtagg ctgtcctctg ggctggtgac ggcggctctc tatgggcgct
1681tgtatgagag ggactaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1741ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1801cgacaatcaa cctctggatt a

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