Construct: ORF ccsbBroad304_00191
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004822.1_s304c1, BRDN0000406955
- Derived from:
- ccsbBroadEn_00191
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL49 (740)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00191
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 740 | MRPL49 | mitochondrial ribosomal pro... | NM_004927.4 | 100% | 100% | |
| 2 | human | 740 | MRPL49 | mitochondrial ribosomal pro... | NR_037567.1 | 22.6% | (many diffs) | |
| 3 | human | 740 | MRPL49 | mitochondrial ribosomal pro... | NR_037568.2 | 17.1% | 1_26del;103_104ins151;374_1875del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agctaccatg ttccgggcta cgctgcgggg atggagaacc ggtgtccagc 121 ggggctgcgg gctacggctg ttgagccaga cccagggccc tccagattac cccaggtttg 181 tggagtctgt ggatgaatat cagtttgtgg agcgcctgtt accggctacc aggatcccag 241 atcccccaaa gcatgaacat tatcctaccc ctagtggctg gcagcctccc agagaccccc 301 cacccaacct gccttacttt gtacgacgct ctcggatgca caacatcccc gtctacaagg 361 acatcacgca tggcaaccgg cagatgactg tgatccggaa agtggaaggg gacatctggg 421 ccctgcagaa agacgtggaa gattttctga gcccgctgct ggggaagaca cctgtcaccc 481 aggtcaatga ggtgacaggt accctacgga tcaagggcta ctttgaccag gagcttaaag 541 cctggctctt ggagaaaggc ttctgcccaa ctttcttgta caaagtggtt ggtaagccta 601 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 661 gcgttaagtc gacaatcaac ctctggatta