Transcript: Human NM_004927.4

Homo sapiens mitochondrial ribosomal protein L49 (MRPL49), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
MRPL49 (740)
Length:
2026
CDS:
27..527

Additional Resources:

NCBI RefSeq record:
NM_004927.4
NBCI Gene record:
MRPL49 (740)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004927.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000276508 TGTGGAGTCTGTGGATGAATA pLKO_005 140 CDS 100% 13.200 9.240 N MRPL49 n/a
2 TRCN0000276509 CACCCAACCTGCCTTACTTTG pLKO_005 262 CDS 100% 10.800 7.560 N MRPL49 n/a
3 TRCN0000178727 CCTGGAGATTCCATTCCATTT pLKO.1 1845 3UTR 100% 10.800 7.560 N MRPL49 n/a
4 TRCN0000276564 CCTGGAGATTCCATTCCATTT pLKO_005 1845 3UTR 100% 10.800 7.560 N MRPL49 n/a
5 TRCN0000155725 CTGCAGAAAGACGTGGAAGAT pLKO.1 384 CDS 100% 4.950 3.465 N MRPL49 n/a
6 TRCN0000276565 CTGCAGAAAGACGTGGAAGAT pLKO_005 384 CDS 100% 4.950 3.465 N MRPL49 n/a
7 TRCN0000152189 CCAAAGCATGAACATTATCCT pLKO.1 207 CDS 100% 3.000 2.100 N MRPL49 n/a
8 TRCN0000156198 CCTCTTGAGTAGCTGGGATTA pLKO.1 909 3UTR 100% 10.800 5.400 Y MRPL49 n/a
9 TRCN0000276507 CCTCTTGAGTAGCTGGGATTA pLKO_005 909 3UTR 100% 10.800 5.400 Y MRPL49 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004927.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00191 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00191 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466908 AGTGGCGTCGTCTGATCTGAAAAA pLX_317 87.2% 100% 100% V5 n/a
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