Construct: ORF ccsbBroad304_00196

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012063.1_s304c1, BRDN0000461124
Derived from:
ccsbBroadEn_00196
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM50B (757)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00196
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 757 TMEM50B transmembrane protein 50B NM_006134.7 100% 100%
2 human 757 TMEM50B transmembrane protein 50B XM_011529746.2 100% 100%
3 human 757 TMEM50B transmembrane protein 50B NR_040016.1 15.1% 1_213del;688_3127del
4 mouse 77975 Tmem50b transmembrane protein 50B NM_030018.3 87.7% 98.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
540
ORF length:
474
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc aggcttccta gataattttc gttggccaga atgtgaatgt attgactgga
121gtgagagaag aaatgctgtg gcatctgttg tcgcaggtat attgtttttt acaggctggt
181ggataatgat tgatgcagct gtggtgtatc ctaagccaga acagttgaac catgcctttc
241acacatgtgg tgtattttcc acattggctt tcttcatgat aaatgctgta tccaatgctc
301aggtgagagg tgatagctat gaaagcggct gtttaggaag aacaggtgct cgagtttggc
361ttttcattgg tttcatgttg atgtttgggt cacttattgc ttccatgtgg attctttttg
421gtgcatatgt tacccaaaat actgatgttt atccgggact agctgtgttt tttcaaaatg
481cacttatatt ttttagcact ctgatctaca aatttggaag aaccgaagag ctatggacct
541gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
601attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
661ggatta

Download FASTA (ORF) (Full)