Construct: ORF ccsbBroad304_00227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015394.1_s304c1, BRDN0000406026
- Derived from:
- ccsbBroadEn_00227
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAV2 (858)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00227
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 858 | CAV2 | caveolin 2 | NM_001233.5 | 100% | 100% | |
| 2 | human | 858 | CAV2 | caveolin 2 | NM_001206747.2 | 91.9% | 91.9% | 0_1ins39 |
| 3 | human | 858 | CAV2 | caveolin 2 | NM_198212.3 | 56.8% | 38.2% | 149_150ins188;299_336del |
| 4 | human | 858 | CAV2 | caveolin 2 | NM_001206748.2 | 48.7% | 48.7% | 0_1ins249 |
| 5 | mouse | 12390 | Cav2 | caveolin 2 | NM_016900.4 | 86.6% | 90.1% | (many diffs) |
| 6 | mouse | 12390 | Cav2 | caveolin 2 | NM_001277756.1 | 60.9% | 62.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gctggagacg gagaaggcgg acgtacagct cttcatggac gacgactcct 121 acagccacca cagcggcctc gagtacgccg accccgagaa gttcgcggac tcggaccagg 181 accgggatcc ccaccggctc aactcgcatc tcaagctggg cttcgaggat gtgatcgcag 241 agccggtgac tacgcactcc tttgacaaag tgtggatctg cagccatgcc ctctttgaaa 301 tcagcaaata cgtaatgtac aagttcctga cggtgttcct ggccattccc ctggccttca 361 ttgcgggaat tctctttgcc accctcagct gtctgcacat ctggatttta atgccttttg 421 taaagacctg cctaatggtt ctgccttcag tgcagacaat atggaagagt gtgacagatg 481 ttatcattgc tccattgtgt acgagcgtag gacgatgctt ctcttctgtc agcctgcaac 541 tgagccagga ttgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 601 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 661 caatcaacct ctggatta