Construct: ORF ccsbBroad304_00257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004276.1_s304c1, BRDN0000406748
- Derived from:
- ccsbBroadEn_00257
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCARB2 (950)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00257
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 950 | SCARB2 | scavenger receptor class B ... | NM_005506.4 | 100% | 100% | |
| 2 | human | 950 | SCARB2 | scavenger receptor class B ... | NM_001204255.2 | 70% | 70% | 275_276ins429 |
| 3 | mouse | 12492 | Scarb2 | scavenger receptor class B,... | NM_007644.4 | 84.5% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ccgatgctgc ttctacacgg cggggacgtt gtccctgctc ctgctggtga 121 ccagcgtcac gctgctggtg gcccgggtct tccagaaggc tgtagaccag agtatcgaga 181 agaaaattgt gttaaggaat ggtactgagg catttgactc ctgggagaag ccccctctgc 241 ctgtgtatac tcagttctat ttcttcaatg tcaccaatcc agaggagatc ctcagagggg 301 agacccctcg ggtggaagaa gtggggccat acacctacag ggaactcaga aacaaagcaa 361 atattcaatt tggagataat ggaacaacaa tatctgctgt tagcaacaag gcctatgttt 421 ttgaacgaga ccaatctgtt ggagacccta aaattgactt aattagaaca ttaaatattc 481 ctgtattgac tgtcatagag tggtcccagg tgcacttcct cagggagatc atcgaggcca 541 tgttgaaagc ctatcagcag aagctctttg tgactcacac agttgacgaa ttgctctggg 601 gctacaaaga tgaaatcttg tcccttatcc atgttttcag gcccgatatc tctccctatt 661 ttggcctatt ctatgagaaa aatgggacta atgatggaga ctatgttttt ctaactggag 721 aagacagtta ccttaacttt acaaaaattg tggaatggaa tgggaaaacg tcacttgact 781 ggtggataac agacaagtgc aatatgatta atggaacaga tggagattct tttcacccac 841 taataaccaa agatgaggtc ctttatgtct tcccatctga cttttgcagg tcagtgtata 901 ttactttcag tgactatgag agtgtacagg gactgcctgc ctttcggtat aaagttcctg 961 cagaaatatt agccaatacg tcagacaatg ccggcttctg tatacctgag ggaaactgcc 1021 tgggctcagg agttctgaat gtcagcatct gcaagaatgg tgcacccatc attatgtctt 1081 tcccacactt ttaccaagca gatgagaggt ttgtttctgc catagaaggc atgcacccaa 1141 atcaggaaga ccatgagaca tttgtggaca ttaatccttt gactggaata atcctaaaag 1201 cagccaagag gttccaaatc aacatttatg tcaaaaaatt agatgacttt gttgaaacgg 1261 gagacattag aaccatggtt ttcccagtga tgtacctcaa tgagagtgtt cacattgata 1321 aagagacggc gagtcgactg aagtctatga ttaacactac tttgatcatc accaacatac 1381 cctacatcat catggcgctg ggtgtgttct ttggtttggt ttttacctgg cttgcatgca 1441 aaggacaggg atccatggat gagggaacag cggatgaaag agcacccctc attcgaacct 1501 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1561 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1621 ggatta