Construct: ORF ccsbBroad304_00281
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011987.1_s304c1, BRDN0000408610
- Derived from:
- ccsbBroadEn_00281
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK8 (1024)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00281
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001260.3 | 100% | 100% | |
| 2 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001318368.2 | 99.7% | 99.7% | 1110_1111insAAG |
| 3 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020317.2 | 72.8% | 72.8% | 0_1ins378 |
| 4 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001346501.2 | 62.7% | 62.7% | 0_1ins519 |
| 5 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_011534865.2 | 62.7% | 62.7% | 0_1ins519 |
| 6 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020318.1 | 62.5% | 62.5% | 0_1ins519;591_592insAAG |
| 7 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_153599.4 | 93.9% | 98.9% | (many diffs) |
| 8 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359990.1 | 93.7% | 98.7% | (many diffs) |
| 9 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504836.4 | 92.8% | 97.8% | (many diffs) |
| 10 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504838.4 | 91.3% | 96.1% | (many diffs) |
| 11 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504839.4 | 86.7% | 91.1% | (many diffs) |
| 12 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504840.4 | 83.6% | 88.5% | (many diffs) |
| 13 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_030254515.1 | 83.4% | 88.3% | (many diffs) |
| 14 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359991.1 | 80.6% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1461
- ORF length:
- 1392
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggactatgac tttaaagtga agctgagcag cgagcgggag cgggtcgagg 121 acctgtttga atacgagggc tgcaaagttg gccgaggcac ttatggtcac gtctacaaag 181 ccaagaggaa agatgggaag gatgataaag actatgcttt aaaacaaata gaaggaactg 241 ggatctctat gtcggcatgt agagaaatag cattacttcg agagcttaag catccaaacg 301 tcatttctct tcaaaaggtg tttctgtctc atgctgatag gaaggtgtgg cttctgtttg 361 actatgctga acatgacctc tggcatataa tcaagtttca cagagcttct aaagcaaaca 421 agaagccagt tcagttacct cggggaatgg tgaagtcact attatatcag atcctagatg 481 gtattcacta cctgcatgct aactgggtgt tgcacagaga tttgaaacct gctaatattt 541 tagttatggg tgaaggtcct gagcgaggaa gagtaaaaat tgctgacatg ggctttgccc 601 gattatttaa ttcacctttg aagcctttag cagatttgga tccagtggtt gttacattct 661 ggtaccgagc ccctgaacta cttcttggag caaggcatta taccaaagct attgatattt 721 gggctatagg gtgtatattt gcagaactac taacgtcaga accaatattt cactgtcgac 781 aagaggacat caaaactagt aatccttatc accatgacca gctggacaga atattcaatg 841 taatgggatt tccTGCAGAT AAAGATTGGG AAGATATAAA AAAGATGCCT GAACATTCAA 901 CATTAATGAA AGATTTCAGA AGAAATACGT ATACCAACTG CAGCCTTATC AAGTATATGG 961 AAAAACATAA AGTTAAACCA GATAGTAAAG CATTCCACTT GCTTCAGAAG CTGCTTACCA 1021 TGGACCCAAT AAAGCGAATT ACCTCAGAAC AGGCTATGCA GGACCCCTAT TTCTTAGAAG 1081 ACCCACTTCC TACATCAGAC GTTTTTGCCG GTTGTCAAAT CCCTTACCCA AAACGAGAAT 1141 TTTTAACGGA AGAAGAACCT GATGACAAAG GAGACAAAAA GAACCAGCAG CAGCAGCAGG 1201 GCAATAACCA CACTAATGGA ACTGGCCACC CAGGGAATCA AGACAGCAGT CACACACAGG 1261 GACCCCCGTT GAAGAAAGTG AGAGTTGTTC CTCCTACCAC TACCTCAGGT GGACTTATCA 1321 TGACCTCAGA CTATCAGCGT TCCAATCCAC ATGCTGCCTA TCCCAACCCT GGACCAAGCA 1381 CATCACAGCC GCAGAGCAGC ATGGGATACT CAGCTACCTC CCAGCAGCCT CCACAGTACT 1441 CACATCAGAC ACATCGGTAC TTGCCAACTT TCTTGTACaa agtggttggt aagcctatcc 1501 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1561 ttaagtcgac aatcaacctc tggatta