Construct: ORF ccsbBroad304_00281

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011987.1_s304c1, BRDN0000408610
Derived from:
ccsbBroadEn_00281
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDK8 (1024)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00281
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1024 CDK8 cyclin dependent kinase 8 NM_001260.3 100% 100%
2 human 1024 CDK8 cyclin dependent kinase 8 NM_001318368.2 99.7% 99.7% 1110_1111insAAG
3 human 1024 CDK8 cyclin dependent kinase 8 XM_017020317.2 72.8% 72.8% 0_1ins378
4 human 1024 CDK8 cyclin dependent kinase 8 NM_001346501.2 62.7% 62.7% 0_1ins519
5 human 1024 CDK8 cyclin dependent kinase 8 XM_011534865.2 62.7% 62.7% 0_1ins519
6 human 1024 CDK8 cyclin dependent kinase 8 XM_017020318.1 62.5% 62.5% 0_1ins519;591_592insAAG
7 mouse 264064 Cdk8 cyclin-dependent kinase 8 NM_153599.4 93.9% 98.9% (many diffs)
8 mouse 264064 Cdk8 cyclin-dependent kinase 8 NM_001359990.1 93.7% 98.7% (many diffs)
9 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504836.4 92.8% 97.8% (many diffs)
10 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504838.4 91.3% 96.1% (many diffs)
11 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504839.4 86.7% 91.1% (many diffs)
12 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_006504840.4 83.6% 88.5% (many diffs)
13 mouse 264064 Cdk8 cyclin-dependent kinase 8 XM_030254515.1 83.4% 88.3% (many diffs)
14 mouse 264064 Cdk8 cyclin-dependent kinase 8 NM_001359991.1 80.6% 84.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1461
ORF length:
1392
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggactatgac tttaaagtga agctgagcag cgagcgggag cgggtcgagg
121acctgtttga atacgagggc tgcaaagttg gccgaggcac ttatggtcac gtctacaaag
181ccaagaggaa agatgggaag gatgataaag actatgcttt aaaacaaata gaaggaactg
241ggatctctat gtcggcatgt agagaaatag cattacttcg agagcttaag catccaaacg
301tcatttctct tcaaaaggtg tttctgtctc atgctgatag gaaggtgtgg cttctgtttg
361actatgctga acatgacctc tggcatataa tcaagtttca cagagcttct aaagcaaaca
421agaagccagt tcagttacct cggggaatgg tgaagtcact attatatcag atcctagatg
481gtattcacta cctgcatgct aactgggtgt tgcacagaga tttgaaacct gctaatattt
541tagttatggg tgaaggtcct gagcgaggaa gagtaaaaat tgctgacatg ggctttgccc
601gattatttaa ttcacctttg aagcctttag cagatttgga tccagtggtt gttacattct
661ggtaccgagc ccctgaacta cttcttggag caaggcatta taccaaagct attgatattt
721gggctatagg gtgtatattt gcagaactac taacgtcaga accaatattt cactgtcgac
781aagaggacat caaaactagt aatccttatc accatgacca gctggacaga atattcaatg
841taatgggatt tccTGCAGAT AAAGATTGGG AAGATATAAA AAAGATGCCT GAACATTCAA
901CATTAATGAA AGATTTCAGA AGAAATACGT ATACCAACTG CAGCCTTATC AAGTATATGG
961AAAAACATAA AGTTAAACCA GATAGTAAAG CATTCCACTT GCTTCAGAAG CTGCTTACCA
1021TGGACCCAAT AAAGCGAATT ACCTCAGAAC AGGCTATGCA GGACCCCTAT TTCTTAGAAG
1081ACCCACTTCC TACATCAGAC GTTTTTGCCG GTTGTCAAAT CCCTTACCCA AAACGAGAAT
1141TTTTAACGGA AGAAGAACCT GATGACAAAG GAGACAAAAA GAACCAGCAG CAGCAGCAGG
1201GCAATAACCA CACTAATGGA ACTGGCCACC CAGGGAATCA AGACAGCAGT CACACACAGG
1261GACCCCCGTT GAAGAAAGTG AGAGTTGTTC CTCCTACCAC TACCTCAGGT GGACTTATCA
1321TGACCTCAGA CTATCAGCGT TCCAATCCAC ATGCTGCCTA TCCCAACCCT GGACCAAGCA
1381CATCACAGCC GCAGAGCAGC ATGGGATACT CAGCTACCTC CCAGCAGCCT CCACAGTACT
1441CACATCAGAC ACATCGGTAC TTGCCAACTT TCTTGTACaa agtggttggt aagcctatcc
1501ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1561ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)