Construct: ORF ccsbBroad304_00304

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008827.1_s304c1, BRDN0000401595
Derived from:
ccsbBroadEn_00304
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRM2 (1129)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00304
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1129 CHRM2 cholinergic receptor muscar... NM_000739.2 100% 100%
2 human 1129 CHRM2 cholinergic receptor muscar... NM_001006626.2 100% 100%
3 human 1129 CHRM2 cholinergic receptor muscar... NM_001006627.2 100% 100%
4 human 1129 CHRM2 cholinergic receptor muscar... NM_001006628.2 100% 100%
5 human 1129 CHRM2 cholinergic receptor muscar... NM_001006629.2 100% 100%
6 human 1129 CHRM2 cholinergic receptor muscar... NM_001006630.1 100% 100%
7 human 1129 CHRM2 cholinergic receptor muscar... NM_001006631.2 100% 100%
8 human 1129 CHRM2 cholinergic receptor muscar... NM_001006632.2 100% 100%
9 human 1129 CHRM2 cholinergic receptor muscar... XM_024446648.1 100% 100%
10 mouse 243764 Chrm2 cholinergic receptor, musca... NM_203491.3 89.3% 96.3% (many diffs)
11 mouse 243764 Chrm2 cholinergic receptor, musca... XM_006506136.2 89.3% 96.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1467
ORF length:
1398
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaataactca acaaactcct ctaacaatag cctggctctt acaagtcctt
121ataagacatt tgaagtggtg tttattgtcc tggtggctgg atccctcagt ttggtgacca
181ttatcgggaa catcctagtc atggtttcca ttaaagtcaa ccgccacctc cagaccgtca
241acaattactt tttattcagc ttggcctgtg ctgaccttat cataggtgtt ttctccatga
301acttgtacac cctctacact gtgattggtt actggccttt gggacctgtg gtgtgtgacc
361tttggctagc cctggactat gtggtcagca atgcctcagt tatgaatctg ctcatcatca
421gctttgacag gtacttctgt gtcacaaaac ctctgaccta cccagtcaag cggaccacaa
481aaatggcagg tatgatgatt gcagctgcct gggtcctctc tttcatcctc tgggctccag
541ccattctctt ctggcagttc attgtagggg tgagaactgt ggaggatggg gagtgctaca
601ttcagttttt ttccaatgct gctgtcacct ttggtacggc tattgcagcc ttctatttgc
661cagtgatcat catgactgtg ctatattggc acatatcccg agccagcaag agcaggataa
721agaaggacaa gaaggagcct gttgccaacc aagaccccgt ttctccaagt ctggtacaag
781gaaggatagt gaagccaaac aataacaaca tgcccagcag tgacgatggc ctggagcaca
841acaaaatcca gaatggcaaa gcccccaggg atcctgtgac tgaaaactgt gttcagggag
901aggagaagga gagctccaat gactccacct cagtcagtgc tgttgcctct aatatgagag
961atgatgaaat aacccaggat gaaaacacag tttccacttc cctgggccat tccaaagatg
1021agaactctaa gcaaacatgc atcagaattg gcaccaagac cccaaaaagt gactcatgta
1081ccccaactaa taccaccgtg gaggtagtgg ggtcttcagg tcagaatgga gatgaaaagc
1141agaatattgt agcccgcaag attgtgaaga tgactaagca gcctgcaaaa aagaagcctc
1201ctccttcccg ggaaaagaaa gtcaccagga caatcttggc tattctgttg gctttcatca
1261tcacttgggc cccatacaat gtcatggtgc tcattaacac cttttgtgca ccttgcatcc
1321ccaacactgt gtggacaatt ggttactggc tttgttacat caacagcact atcaaccctg
1381cctgctatgc actttgcaat gccaccttca agaagacctt taaacacctt ctcatgtgtc
1441attataagaa cataggcgct acaaggttgc caactttctt gtacaaagtg gttggtaagc
1501ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1561ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)