Construct: ORF ccsbBroad304_00306

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010790.1_s304c1, BRDN0000461500
Derived from:
ccsbBroadEn_00306
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRM4 (1132)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00306
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1132 CHRM4 cholinergic receptor muscar... NM_000741.5 100% 100%
2 human 1132 CHRM4 cholinergic receptor muscar... NM_001366692.1 100% 100%
3 mouse 12672 Chrm4 cholinergic receptor, musca... NM_007699.2 89.3% 95.8% (many diffs)
4 mouse 12672 Chrm4 cholinergic receptor, musca... XM_006498654.2 85.9% 92.1% (many diffs)
5 mouse 12672 Chrm4 cholinergic receptor, musca... XM_011239271.2 67.2% 72.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1506
ORF length:
1437
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggccaacttc acacctgtca atggcagctc gggcaatcag tccgtgcgcc
121tggtcacgtc atcatcccac aatcgctatg agacggtgga aatggtcttc attgccacag
181tgacaggctc cctgagcctg gtgactgtcg tgggcaacat cctggtgatg ctgtccatca
241aggtcaacag gcagctgcag acagtcaaca actacttcct cttcagcctg gcgtgtgctg
301atctcatcat aggcgccttc tccatgaacc tctacaccgt gtacatcatc aagggctact
361ggcccctggg cgccgtggtc tgcgacctgt ggctggccct ggactacgtg gtgagcaacg
421cctccgtcat gaaccttctc atcatcagct ttgaccgcta cttctgcgtc accaagcctc
481tcacctaccc tgcccggcgc accaccaaga tggcaggcct catgattgct gctgcctggg
541tactgtcctt cgtgctctgg gcgcctgcca tcttgttctg gcagtttgtg gtgggtaagc
601ggacggtgcc cgacaaccag tgcttcatcc agttcctgtc caacccagca gtgacctttg
661gcacagccat tgctgccttc tacctgcctg tggtcatcat gacggtgctg tacatccaca
721tctccctggc cagtcgcagc cgagtccaca agcaccggcc cgagggcccg aaggagaaga
781aagccaagac gctggccttc ctcaagagcc cactaatgaa gcagagcgtc aagaagcccc
841cgcccgggga ggccgcccgg gaggagctgc gcaatggcaa gctggaggag gcccccccgc
901cagcgctgcc accgccaccg cgccccgtgg ctgataagga cacttccaat gagtccagct
961caggcagtgc cacccagaac accaaggaac gcccagccac agagctgtcc accacagagg
1021ccaccacgcc cgccatgccc gcccctcccc tgcagccgcg ggccctcaac ccagcctcca
1081gatggtccaa gatccagatt gtgacgaagc agacaggcaa tgagtgtgtg acagccattg
1141agattgtgcc tgccacgccg gctggcatgc gccctgcggc caacgtggcc cgcaagttcg
1201ccagcatcgc tcgcaaccag gtgcgcaaga agcggcagat ggcggcccgg gagcgcaaag
1261tgacacgaac gatctttgcc attctgctag ccttcatcct cacctggacg ccctacaacg
1321tcatggtcct ggtgaacacc ttctgccaga gctgcatccc tgacacggtg tggtccattg
1381gctactggct ctgctacgtc aacagcacca tcaaccctgc ctgctatgct ctgtgcaacg
1441ccacctttaa aaagaccttc cggcacctgc tgctgtgcca gtatcggaac atcggcactg
1501ccaggttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1561gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1621acctctggat ta

Download FASTA (ORF) (Full)