Transcript: Mouse NM_007699.2

Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Chrm4 (12672)
Length:
2768
CDS:
188..1627

Additional Resources:

NCBI RefSeq record:
NM_007699.2
NBCI Gene record:
Chrm4 (12672)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007699.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419541 ATCATGACGGTGCTGTATATT pLKO_005 812 CDS 100% 15.000 10.500 N Chrm4 n/a
2 TRCN0000414310 TCTGATGAAGCCGAGCATTAA pLKO_005 928 CDS 100% 13.200 9.240 N Chrm4 n/a
3 TRCN0000367729 CCATCTTGTTCTGGCAGTTTG pLKO_005 684 CDS 100% 10.800 7.560 N CHRM4 n/a
4 TRCN0000026322 CCAAGATCCAAATTGTGACAA pLKO.1 1206 CDS 100% 4.950 3.465 N Chrm4 n/a
5 TRCN0000026242 CCAGTGCTTCATCCAGTTCTT pLKO.1 733 CDS 100% 4.950 3.465 N Chrm4 n/a
6 TRCN0000026262 CTGGACTATGTGGTGAGCAAT pLKO.1 515 CDS 100% 4.950 3.465 N Chrm4 n/a
7 TRCN0000026289 GCAGTTGCAGACAGTCAACAA pLKO.1 367 CDS 100% 4.950 3.465 N Chrm4 n/a
8 TRCN0000026300 GCTGACAAGGACACTTCCAAT pLKO.1 1046 CDS 100% 4.950 3.465 N Chrm4 n/a
9 TRCN0000011264 GCCATCTTGTTCTGGCAGTTT pLKO.1 683 CDS 100% 4.950 2.970 N CHRM4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007699.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00306 pDONR223 100% 89.3% 95.8% None (many diffs) n/a
2 ccsbBroad304_00306 pLX_304 0% 89.3% 95.8% V5 (many diffs) n/a
3 TRCN0000476559 CTCTCTAGGCTCAGGCAAATTATA pLX_317 25% 89.3% 95.8% V5 (many diffs) n/a
4 TRCN0000488527 GTCATGATTAGTTTTATACGATGT pLX_317 23.5% 89.3% 95.8% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000491288 TAGGGCGCTACTTAGAGATGACAG pLX_317 23.4% 89.2% 95.6% V5 (many diffs) n/a
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