Construct: ORF ccsbBroad304_00310

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009914.1_s304c1, BRDN0000462368
Derived from:
ccsbBroadEn_00310
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRNB4 (1143)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00310
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1143 CHRNB4 cholinergic receptor nicoti... NM_000750.5 100% 100%
2 human 1143 CHRNB4 cholinergic receptor nicoti... XM_011521186.2 95.9% 95.6% (many diffs)
3 human 1143 CHRNB4 cholinergic receptor nicoti... XM_011521187.2 95.9% 95.6% (many diffs)
4 human 1143 CHRNB4 cholinergic receptor nicoti... XM_017021885.1 93.9% 93.9% 0_1ins90
5 human 1143 CHRNB4 cholinergic receptor nicoti... XM_017021886.1 93.9% 93.9% 0_1ins90
6 human 1143 CHRNB4 cholinergic receptor nicoti... XM_017021887.1 91.7% 88.1% (many diffs)
7 human 1143 CHRNB4 cholinergic receptor nicoti... XM_017021888.1 90.3% 88.5% (many diffs)
8 human 1143 CHRNB4 cholinergic receptor nicoti... XM_017021889.2 89.8% 89.5% (many diffs)
9 human 1143 CHRNB4 cholinergic receptor nicoti... XM_011521190.2 85.1% 85.1% 0_1ins222
10 human 1143 CHRNB4 cholinergic receptor nicoti... XM_011521191.2 85.1% 85.1% 0_1ins222
11 human 1143 CHRNB4 cholinergic receptor nicoti... XM_011521192.2 63.4% 63.4% 0_1ins546
12 mouse 108015 Chrnb4 cholinergic receptor, nicot... NM_148944.4 83% 82.2% (many diffs)
13 mouse 108015 Chrnb4 cholinergic receptor, nicot... XM_006510757.3 78.9% 78.6% (many diffs)
14 mouse 108015 Chrnb4 cholinergic receptor, nicot... XM_011242655.2 78.9% 78.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1563
ORF length:
1494
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaggcgcgcg ccttccctgg tccttttctt cctggtcgcc ctttgcgggc
121gcgggaactg ccgcgtggcc aatgcggagg aaaagctgat ggacgacctt ctgaacaaaa
181cccgttacaa taacctgatc cgcccagcca ccagctcctc acagctcatc tccatcaagc
241tgcagctctc cctggcccag cttatcagcg tgaatgagcg agagcagatc atgaccacca
301atgtctggct gaaacaggaa tggactgatt accgcctgac ctggaacagc tcccgctacg
361agggtgtgaa catcctgagg atccctgcaa agcgcatctg gttgcctgac atcgtgcttt
421acaacaacgc cgacgggacc tatgaggtgt ctgtctacac caacttgata gtccggtcca
481acggcagcgt cctgtggctg ccccctgcca tctacaagag cgcctgcaag attgaggtga
541agtactttcc cttcgaccag cagaactgca ccctcaagtt ccgctcctgg acctatgacc
601acacggagat agacatggtc ctcatgacgc ccacagccag catggatgac tttactccca
661gtggtgagtg ggacatagtg gccctcccag ggagaaggac agtgaaccca caagacccca
721gctacgtgga cgtgacttac gacttcatca tcaagcgcaa gcctctgttc tacaccatca
781acctcatcat cccctgcgtg ctcaccacct tgctggccat cctcgtcttc tacctgccat
841ccgactgcgg cgagaagatg acactgtgca tctcagtgct gctggcactg acattcttcc
901tgctgctcat ctccaagatc gtgccaccca cctccctcga tgtgcctctc atcggcaagt
961acctcatgtt caccatggtg ctggtcacct tctccatcgt caccagcgtc tgtgtgctca
1021atgtgcacca ccgctcgccc agcacccaca ccatggcacc ctgggtcaag cgctgcttcc
1081tgcacaagct gcctaccttc ctcttcatga agcgccctgg ccccgacagc agcccggcca
1141gagccttccc gcccagcaag tcatgcgtga ccaagcccga ggccaccgcc acctccacca
1201gcccctccaa cttctatggg aactccatgt actttgtgaa ccccgcctct gcagcttcca
1261agtctccagc cggctctacc ccggtggcta tccccaggga tttctggctg cggtcctctg
1321ggaggttccg acaggatgtg caggaggcat tagaaggtgt cagcttcatc gcccagcaca
1381tgaagaatga cgatgaagac cagagtgtcg ttgaggactg gaagtacgtg gctatggtgg
1441tggaccggct gttcctgtgg gtgttcatgt ttgtgtgcgt cctgggcact gtggggctct
1501tcctaccgcc cctcttccag acccatgcag cttctgaggg gccctacgct gcccagcgtg
1561acttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1621tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1681tctggatta

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