Construct: ORF ccsbBroad304_00416

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003396.1_s304c1, BRDN0000400825
Derived from:
ccsbBroadEn_00416
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CYP27A1 (1593)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00416
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1593 CYP27A1 cytochrome P450 family 27 s... NM_000784.4 100% 100%
2 human 1593 CYP27A1 cytochrome P450 family 27 s... XM_017003488.2 73.6% 71.5% 0_1ins229;26_27ins191
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1659
ORF length:
1593
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tgcgctgggc tgcgcgaggc tgaggtgggc gctgcgaggg gccggccgtg
121gcctctgccc ccacggggcc agagccaagg ccgcgatccc tgccgccctc ccctcggaca
181aggccaccgg agctcccgga gccgggcctg gtgtccggcg gcggcaacgg agcttagagg
241agattccacg tctaggacag ctgcgcttct tctttcagct gttcgttcaa ggctatgccc
301tgcaactgca ccagttacag gtgctttaca aggccaagta cggtccaatg tggatgtcct
361acttagggcc tcagatgcac gtgaacctgg ccagtgcccc gctcttggag caagtgatgc
421ggcaagaggg caagtaccca gtacggaacg acatggagct atggaaggag caccgggacc
481agcacgacct gacctatggg ccgttcacca cggaaggaca ccactggtac cagctgcgcc
541aggctctgaa ccagcggttg ctgaagccag cggaagcagc gctctatacg gatgctttca
601atgaggtgat tgatgacttt atgactcgac tggaccagct gcgggcagag agtgcttcgg
661ggaaccaggt gtcggacatg gctcaactct tctactactt tgccttggaa gctatttgct
721acatcctgtt cgagaaacgc attggctgcc tgcagcgatc catccccgag gacaccgtga
781ccttcgtcag atccatcggg ttaatgttcc agaactcact ctatgccacc ttcctcccca
841agtggactcg ccccgtgctg cctttctgga agcgatacct ggatggttgg aatgccatct
901tttcctttgg gaagaagctg attgatgaga agctcgaaga tatggaggcc caactgcagg
961cagcagggcc agatggcatc caggtgtctg gctacctgca cttcttactg gccagtggac
1021agctcagtcc tcgggaggcc atgggcagcc tgcctgagct gctcatggct ggagtggaca
1081cgacatccaa cacgctgaca tgggccctgt accacctctc aaaggaccct gagatccagg
1141aggccttgca cgaggaagtg gtgggtgtgg tgccagccgg gcaagtgccc cagcacaagg
1201actttgccca catgccgttg ctcaaagctg tgcttaagga gactctgcgt ctctaccctg
1261tggtccccac aaactcccgg atcatagaaa aggaaattga agttgatggc ttcctcttcc
1321ccaagaacac ccagtttgtg ttctgccact atgtggtgtc ccgggacccc actgccttct
1381ctgagcctga aagcttccag ccccaccgct ggctgagaaa cagccagcct gctaccccca
1441ggatccagca cccatttggc tctgtgccct ttggctatgg ggtccgggcc tgcctgggcc
1501gcaggattgc agagctggag atgcagctac tcctcgcaag gctgatccag aagtacaagg
1561tggtcctggc cccggagacg ggggagttga agagtgtggc ccgcattgtc ctggttccca
1621ataagaaagt gggcctgcag ttcctgcaga gacagtgctg cccaactttc ttgtacaaag
1681tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1741gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)