Transcript: Human XM_017003488.2

PREDICTED: Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CYP27A1 (1593)
Length:
2118
CDS:
680..1855

Additional Resources:

NCBI RefSeq record:
XM_017003488.2
NBCI Gene record:
CYP27A1 (1593)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003488.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231147 AGCAACTCCCTCTCAGCTAAA pLKO_005 2027 3UTR 100% 10.800 7.560 N CYP27A1 n/a
2 TRCN0000231146 CCACAAACTCCCGGATCATAG pLKO_005 1461 CDS 100% 10.800 7.560 N CYP27A1 n/a
3 TRCN0000231145 TCGTCAGATCCATCGGGTTAA pLKO_005 978 CDS 100% 10.800 7.560 N CYP27A1 n/a
4 TRCN0000062160 CGATACCTGGATGGTTGGAAT pLKO.1 1067 CDS 100% 4.950 3.465 N CYP27A1 n/a
5 TRCN0000062162 CGCATTGTCCTGGTTCCCAAT pLKO.1 1796 CDS 100% 4.050 2.835 N CYP27A1 n/a
6 TRCN0000062158 GCTTTCAATGAGGTGATTGAT pLKO.1 788 CDS 100% 0.563 0.394 N CYP27A1 n/a
7 TRCN0000218094 ACTTTGCCTTGGAAGCTATTT pLKO_005 891 CDS 100% 13.200 7.920 N CYP27A1 n/a
8 TRCN0000062161 CAGTTTGTGTTCTGCCACTAT pLKO.1 1526 CDS 100% 4.950 2.970 N CYP27A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003488.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00416 pDONR223 100% 73.6% 71.5% None 0_1ins229;26_27ins191 n/a
2 ccsbBroad304_00416 pLX_304 0% 73.6% 71.5% V5 0_1ins229;26_27ins191 n/a
3 TRCN0000467534 CCTAACCTGGAGCCGCCGGTATAT pLX_317 25.1% 73.6% 71.5% V5 0_1ins229;26_27ins191 n/a
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