Construct: ORF ccsbBroad304_00428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013764.1_s304c1, BRDN0000410097
- Derived from:
- ccsbBroadEn_00428
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCX (1641)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00428
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1641 | DCX | doublecortin | NM_178151.2 | 100% | 100% | |
| 2 | human | 1641 | DCX | doublecortin | NM_178153.3 | 100% | 100% | |
| 3 | human | 1641 | DCX | doublecortin | NM_001369372.1 | 99.7% | 99.7% | 1027_1029delGTA |
| 4 | human | 1641 | DCX | doublecortin | NM_001369371.1 | 98.6% | 98.6% | 931_945del |
| 5 | human | 1641 | DCX | doublecortin | NM_178152.3 | 98.6% | 98.6% | 931_945del |
| 6 | human | 1641 | DCX | doublecortin | NM_001195553.2 | 98.3% | 98.3% | 931_945del;1042_1044delGTA |
| 7 | human | 1641 | DCX | doublecortin | NM_001369370.1 | 98.3% | 98.3% | 931_945del;1042_1044delGTA |
| 8 | human | 1641 | DCX | doublecortin | XM_011530879.3 | 89.6% | 85.9% | (many diffs) |
| 9 | human | 1641 | DCX | doublecortin | NM_001369373.1 | 88.6% | 86.6% | 929_930ins95;957_958ins28 |
| 10 | human | 1641 | DCX | doublecortin | NM_001369374.1 | 88.6% | 86.6% | 929_930ins95;957_958ins28 |
| 11 | human | 1641 | DCX | doublecortin | NM_000555.3 | 81.6% | 81.6% | 1_243del |
| 12 | mouse | 13193 | Dcx | doublecortin | NM_010025.2 | 89.7% | 99.4% | (many diffs) |
| 13 | mouse | 13193 | Dcx | doublecortin | XM_006528698.3 | 89.4% | 99.1% | (many diffs) |
| 14 | mouse | 13193 | Dcx | doublecortin | NM_001110224.1 | 88.4% | 98% | (many diffs) |
| 15 | mouse | 13193 | Dcx | doublecortin | NM_001110222.1 | 88.2% | 97.8% | (many diffs) |
| 16 | mouse | 13193 | Dcx | doublecortin | NM_001110223.1 | 88.2% | 97.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga acttgatttt ggacactttg acgaaagaga taagacatcc aggaacatgc 121 gaggctcccg gatgaatggg ttgcctagcc ccactcacag cgcccactgt agcttctacc 181 gaaccagaac cttgcaggca ctgagtaatg agaagaaagc caagaaggta cgtttctacc 241 gcaatgggga ccgctacttc aaggggattg tgtacgctgt gtcctctgac cgttttcgca 301 gctttgacgc cttgctggct gacctgacgc gatctctgtc tgacaacatc aacctgcctc 361 agggagtgcg ttacatttac accattgatg gatccaggaa gatcggaagc atggatgaac 421 tggaggaagg ggaaagctat gtctgttcct cagacaactt ctttaaaaag gtggagtaca 481 ccaagaatgt caatcccaac tggtctgtca acgtaaaaac atctgccaat atgaaagccc 541 cccagtcctt ggctagcagc aacagtgcac aggccaggga gaacaaggac tttgtgcgcc 601 ccaagctggt taccatcatc cgcagtgggg tgaagcctcg gaaggctgtg cgtgtgcttc 661 tgaacaagaa gacagcccac tcttttgagc aagtcctcac tgatatcaca gaagccatca 721 aactggagac cggggttgtc aaaaaactct acactctgga tggaaaacag gtaacttgtc 781 tccatgattt ctttggtgat gatgatgtgt ttattgcctg tggtcctgaa aaatttcgct 841 atgctcagga tgatttttct ctggatgaaa atgaatgccg agtcatgaag ggaaacccat 901 cagccacagc tggcccaaag gcatccccaa cacctcagaa gacttcagcc aagagccctg 961 gtcctatgcg ccgaagcaag tctccagctg actcagcaaa cggaacctcc agcagccagc 1021 tctctacccc caagtctaag cagtctccca tctctacgcc caccagtcct ggcagcctcc 1081 ggaagcacaa ggacctgtac ctgcctctgt ccttggatga ctcggactcg cttggtgatt 1141 ccatgtaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1201 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1261 acctctggat ta