Transcript: Mouse NM_010025.2

Mus musculus doublecortin (Dcx), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Dcx (13193)
Length:
8982
CDS:
173..1255

Additional Resources:

NCBI RefSeq record:
NM_010025.2
NBCI Gene record:
Dcx (13193)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_010025.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000105976 CGCGTGCTTCTCAACAAGAAA pLKO.1 758 CDS 100% 5.625 7.875 N Dcx n/a
2 TRCN0000105975 CGGCTGTAATTGGTGGTTGTA pLKO.1 7934 3UTR 100% 4.950 3.960 N Dcx n/a
3 TRCN0000105977 CCCAAACTTGTGACCATCATT pLKO.1 707 CDS 100% 5.625 3.938 N Dcx n/a
4 TRCN0000105979 TCCGCTATGCTCAAGATGATT pLKO.1 942 CDS 100% 5.625 3.938 N Dcx n/a
5 TRCN0000083227 CCAACTGGTCTGTCAACGTAA pLKO.1 603 CDS 100% 4.950 3.465 N DCX n/a
6 TRCN0000105978 GCAGGCATTAAGTAATGAGAA pLKO.1 301 CDS 100% 4.950 3.465 N Dcx n/a
7 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3996 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_010025.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00428 pDONR223 100% 89.7% 99.4% None (many diffs) n/a
2 ccsbBroad304_00428 pLX_304 0% 89.7% 99.4% V5 (many diffs) n/a
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