Construct: ORF ccsbBroad304_00439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010925.1_s304c1, BRDN0000402588
- Derived from:
- ccsbBroadEn_00439
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMM8A (1678)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00439
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1678 | TIMM8A | translocase of inner mitoch... | NM_004085.4 | 100% | 100% | |
| 2 | human | 1678 | TIMM8A | translocase of inner mitoch... | NM_001145951.2 | 48.2% | 45.3% | 134_135delTTinsAGAAGTGCATGGACAAG;140delT;144_145ins133 |
| 3 | mouse | 30058 | Timm8a1 | translocase of inner mitoch... | NM_013898.3 | 90.4% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 357
- ORF length:
- 291
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ttcctcctcc tcttcctccg cggcgggttt gggtgcagtg gacccgcagt 121 tgcagcattt catcgaggta gagactcaaa agcagcgctt ccagcagctg gtgcaccaga 181 tgactgaact ttgttgggag aagtgcatgg acaagcctgg gccaaagttg gacagtcggg 241 ctgaggcctg ttttgtgaac tgcgttgagc gcttcattga tacaagccag ttcatcttga 301 atcgactgga acagacccag aaatccaagc cagttttctc agaaagcctt tctgactgcc 361 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 421 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 481 tta