Construct: ORF ccsbBroad304_00490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011516.1_s304c1, BRDN0000402654
- Derived from:
- ccsbBroadEn_00490
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF4EBP1 (1978)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00490
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1978 | EIF4EBP1 | eukaryotic translation init... | NM_004095.4 | 100% | 100% | |
| 2 | mouse | 13685 | Eif4ebp1 | eukaryotic translation init... | NM_007918.3 | 89.8% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcATGTC CGGGGGCAGC AGCTGCAGCC AGACCCCAAG CCGGGCCATC CCCGCCACTC 121 GCCGGGTGGT GCTCGGCGAC GGCGTGCAGC TCCCGCCCGG GGACTACAGC ACGACCCCCG 181 GCGGCACGCT CTTCAGCACC ACCCCGGGAG GTACCAGGAT CATCTATGAC CGGAAATTCC 241 TGATGGAGTG TCGGAACTCA CCTGTGACCA AAACACCCCC AAGGGATCTG CCCACCATTC 301 CGGGGGTCAC CAGCCCTTCC AGTGATGAGC CCCCCATGGA AGCCAGCCAG AGCCACCTGC 361 GCAATAGCCC AGAAGATAAG CGGGCGGGCG GTGAAGAGTC ACAGTTTGAG ATGGACATTT 421 ACCCAACTTT CTTGTACaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 481 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 541 ggatta