Construct: ORF ccsbBroad304_00538
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014459.1_s304c1, BRDN0000462234
- Derived from:
- ccsbBroadEn_00538
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FANCB (2187)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00538
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2187 | FANCB | FA complementation group B | NM_001018113.3 | 100% | 100% | |
| 2 | human | 2187 | FANCB | FA complementation group B | NM_001324162.1 | 100% | 100% | |
| 3 | human | 2187 | FANCB | FA complementation group B | NM_152633.3 | 100% | 100% | |
| 4 | human | 2187 | FANCB | FA complementation group B | XM_017029355.2 | 100% | 100% | |
| 5 | human | 2187 | FANCB | FA complementation group B | XM_017029356.1 | 96.5% | 96.5% | (many diffs) |
| 6 | human | 2187 | FANCB | FA complementation group B | XM_011545470.2 | 90.9% | 89.6% | (many diffs) |
| 7 | human | 2187 | FANCB | FA complementation group B | XR_001755674.1 | 54.6% | (many diffs) | |
| 8 | human | 2187 | FANCB | FA complementation group B | XR_001755673.1 | 53.5% | (many diffs) | |
| 9 | human | 2187 | FANCB | FA complementation group B | XR_001755672.1 | 51.3% | (many diffs) | |
| 10 | human | 2187 | FANCB | FA complementation group B | NR_136707.1 | 11.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2646
- ORF length:
- 2577
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gactagcaaa caagcaatgt catctaacga acaagaaagg ctcttgtgtt 121 ataatgggga agtccttgtt ttccagttgt ctaaaggaaa ttttgcagat aaagagccta 181 caaaaacacc catattacat gtcagaagaa tggtatttga cagaggaaca aaagtatttg 241 ttcagaagtc cactggattt tttaccataa aggaagaaaa ctctcattta aaaatcatgt 301 gttgcaactg tgtgtcagat ttcagaactg gaattaacct cccttacatt gtgatagaaa 361 aaaataaaaa gaataatgtt tttgaatatt ttttactaat ccttcacagt actaataaat 421 ttgaaatgcg tttgagtttt aaactaggct atgagatgaa ggatggccta agggtcctta 481 atggcccttt aattttatgg aggcatgtca aagcattctt ctttatctct tctcaaactg 541 gcaaagttgt tagtgtgtca ggtaactttt cctctattca gtgggcaggg gagattgaaa 601 atttaggtat ggttttattg ggactaaagg aatgttgttt atctgaggaa gaatgtactc 661 aagagccttc aaaatcagat tatgcaattt ggaataccaa attttgtgta tattctcttg 721 aaagtcaaga agtattaagt gatatataca ttattcctcc tgcttacagc agtgtggtga 781 cttatgtaca tatttgtgca actgagatca tcaaaaacca gttaagaata tctctcattg 841 cccttactcg aaagaatcag ctgatttcat ttcagaatgg aactcctaaa aatgtgtgcc 901 agcttccatt tggagatcct tgtgcagttc aacttatgga ttcaggtgga ggaaacctct 961 ttttcgttgt atcctttata tccaataatg cttgtgctgt atggaaagag agctttcagg 1021 ttgctgctaa atgggaaaaa cttagcttag tactgataga tgactttatt ggaagtggaa 1081 ctgaacaagt actcctactt tttaaggact ccttgaactc agactgcctg acttcattta 1141 aaataacgga tcttggaaaa ataaactatt cgagtgaacc atcagattgc aatgaagatg 1201 acttatttga agacaaacaa gagaatcgtt acctggtggt tccacctcta gaaacaggac 1261 tgaaagtttg tttttcttct tttcgggaat tacggcagca tctgttgctt aaggaaaaaa 1321 ttatttcaaa atcttacaaa gctttaataa acctagttca aggaaaagat gataatacgt 1381 caagtgcaga ggagaaggaa tgtcttgttc ctctttgtgg tgaagaagaa aattctgtcc 1441 atatcttaga tgaaaagtta tcagacaatt ttcaagattc agaacagcta gtagagaaga 1501 tatggtatcg tgtaatagat gatagcttgg ttgttggagt gaaaactaca tcttctttga 1561 agctgtccct gaatgatgtg actttatcat tgttaatgga tcaagcccat gactccagat 1621 ttcggcttct aaagtgtcaa aatagggtga ttaagttgag tacaaatcct ttcccagcac 1681 catacttgat gccatgtgaa ataggattgg aagcaaaaag ggtcacgttg acccctgata 1741 gcaagaaaga ggaaagcttt gtttgtgaac acccatctaa gaaagagtgt gtacagataa 1801 ttactgctgt aacatctctt tcaccacttt taacattcag taaattttgt tgcactgtac 1861 tgctacaaat tatggagaga gaaagtggta actgtcctaa agatcgttat gttgtgtgtg 1921 gcagagtttt tttaagtcta gaagatcttt caactgggaa gtacctactg acatttccaa 1981 agaagaaacc tatagagcac atggaagatc tttttgcact tcttgcagca ttccataaat 2041 cttgttttca aatcacatca cccggctatg ccctgaattc aatgaaggtg tggctcttag 2101 aacatatgaa atgtgaaata atcaaagaat ttccagaagt gtacttttgt gaaagaccgg 2161 gaagtttcta tgggacactc ttcacttgga aacagagaac accattcgaa gggattttaa 2221 taatctattc caggaatcaa acagttatgt tccagtgcct tcataatctc atcagaattc 2281 tccctataaa ctgtttcctc aaaaatctaa aatcaggaag tgagaatttc ctaattgata 2341 atatggcatt tactttggag aaggaactag tcacccttag ttctctttct tctgccatag 2401 ctaaacatga aagcaatttt atgcagaggt gtgaagtgag caaaggaaag agtagtgtcg 2461 tcgcggctgc tttatcagac agaagggaaa atatccatcc ctacagaaaa gaacttcaga 2521 gagaaaagaa gaaaatgttg caaacgaacc taaaagtgag tggtgccctt tacagagaaa 2581 taactttgaa agtagctgag gttcagttga aatcagactt tgctgcacag aaactgagta 2641 atttattgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2701 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2761 acctctggat ta