Construct: ORF ccsbBroad304_00565
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011115.1_s304c1, BRDN0000409518
- Derived from:
- ccsbBroadEn_00565
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FH (2271)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00565
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2271 | FH | fumarate hydratase | NM_000143.3 | 100% | 100% | |
| 2 | human | 2271 | FH | fumarate hydratase | XM_011544132.2 | 85% | 85% | 0_1ins228 |
| 3 | mouse | 14194 | Fh1 | fumarate hydratase 1 | NM_010209.2 | 87% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1596
- ORF length:
- 1530
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta ccgagcactt cggctcctcg cgcgctcgcg tcccctcgtg cgggctccag 121 ccgcagcctt agcttcggct cccggcttgg gtggcgcggc cgtgccctcg ttttggcctc 181 cgaacgcggc tcgaatggca agccaaaatt ccttccggat agaatatgat acctttggtg 241 aactaaaggt gccaaatgat aagtattatg gcgcccagac cgtgagatct acgatgaact 301 ttaagattgg aggtgtgaca gaacgcatgc caaccccagt tattaaagct tttggcatct 361 tgaagcgagc ggccgctgaa gtaaaccagg attatggtct tgatccaaag attgctaatg 421 caataatgaa ggcagcagat gaggtagctg aaggtaaatt aaatgatcat tttcctctcg 481 tggtatggca gactggatca ggaactcaga caaatatgaa tgtaaatgaa gtcattagca 541 atagagcaat tgaaatgtta ggaggtgaac ttggcagcaa gatacctgtg catcccaacg 601 atcatgttaa taaaagccag agctcaaatg atacttttcc cacagcaatg cacattgctg 661 ctgcaataga agttcatgaa gtactgttac caggactaca gaagttacat gatgctcttg 721 atgcaaaatc caaagagttt gcacagatca tcaagattgg acgtactcat actcaggatg 781 ctgttccact tactcttggg caggaattta gtggttatgt tcaacaagta aaatatgcaa 841 tgacaagaat aaaagctgcc atgccaagaa tctatgagct cgcagctgga ggcactgctg 901 ttggtacagg tttaaatact aGAATTGGCT TTGCAGAAAA GGTTGCTGCA AAAGTGGCTG 961 CACTTACAGG CTTGCCTTTT GTCACTGCTC CGAATAAATT TGAAGCTCTG GCTGCTCATG 1021 ACGCTCTGGT TGAGCTCAGT GGAGCCATGA ACACTACTGC CTGCAGTCTG ATGAAGATAG 1081 CAAATGATAT TCGATTTTTG GGTTCTGGTC CTCGGTCAGG TCTGGGAGAA TTGATCTTGC 1141 CTGAAAATGA ACCAGGAAGC AGTATCATGC CAGGCAAGGT GAACCCTACT CAGTGTGAAG 1201 CAATGACCAT GGTTGCAGCC CAAGTCATGG GGAACCATGT TGCTGTCACT GTCGGAGGCA 1261 GCAATGGACA TTTTGAGTTG AATGTTTTCA AGCCAATGAT GATTAAAAAT GTGTTACACT 1321 CAGCCAGGCT GCTGGGGGAT GCTTCAGTTT CCTTTACAGA AAACTGCGTG GTGGGAATCC 1381 AGGCCAATAC AGAAAGGATC AACAAGCTGA TGAATGAGTC TCTAATGTTG GTGACAGCTC 1441 TCAATCCTCA TATAGGGTAT GACAAGGCAG CAAAGATTGC TAAGACAGCA CACAAAAATG 1501 GATCAACCTT AAAGGAAACT GCTATCGAAC TTGGCTATCT CACAGCAGAG CAGTTTGACG 1561 AATGGGTAAA ACCTAAGGAC ATGCTGGGTC CAAAGTGCCC AACTTTCTTG TACaaagtgg 1621 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1681 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta