Construct: ORF ccsbBroad304_00577

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005515.1_s304c1, BRDN0000411541
Derived from:
ccsbBroadEn_00577
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FOXO3 (2309)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00577
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2309 FOXO3 forkhead box O3 NM_001455.4 100% 100%
2 human 2309 FOXO3 forkhead box O3 NM_201559.3 100% 100%
3 human 2309 FOXO3 forkhead box O3 XM_011535626.2 73.2% 71% (many diffs)
4 human 2309 FOXO3 forkhead box O3 XM_005266867.4 70.4% 69.7% (many diffs)
5 human 2309 FOXO3 forkhead box O3 XM_017010585.1 70.4% 69.7% (many diffs)
6 human 2309 FOXO3 forkhead box O3 XM_005266868.3 67.3% 67.3% 0_1ins660
7 human 2309 FOXO3 forkhead box O3 XM_011535628.3 67.3% 67.3% 0_1ins660
8 human 2309 FOXO3 forkhead box O3 XM_011535629.2 67.3% 67.3% 0_1ins660
9 human 2309 FOXO3 forkhead box O3 XM_017010586.1 67.3% 67.3% 0_1ins660
10 human 2310 FOXO3B forkhead box O3B NM_001368134.1 26.7% 26.2% (many diffs)
11 human 2310 FOXO3B forkhead box O3B NM_001368135.1 26.7% 26.2% (many diffs)
12 mouse 56484 Foxo3 forkhead box O3 NM_019740.2 90.3% 94.6% (many diffs)
13 mouse 56484 Foxo3 forkhead box O3 XM_006512806.1 90.3% 94.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2085
ORF length:
2019
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agaggcaccg gcttccccgg ccccgctctc tccgctcgaa gtggagctgg
121acccggagtt cgagccccag agccgtccgc gatcctgtac gtggcccctg caaaggccgg
181agctccaagc gagccctgcc aagccctcgg gggagacggc cgccgactcc atgatccccg
241aggaggagga cgatgaagac gacgaggacg gcgggggacg ggccggctcg gccatggcga
301tcggcggcgg cggcgggagc ggcacgctgg gctccgggct gctccttgag gactcggccc
361gggtgctggc acccggaggg caagaccccg ggtctgggcc agccaccgcg gcgggcgggc
421tgagcggggg tacacaggcg ctgctgcagc ctcagcaacc gctgccaccg ccgcagccgg
481gggcggctgg gggctccggg cagccgagga aatgttcgtc gcggcggaac gcctggggaa
541acctgtccta cgcggacctg atcacccgcg ccatcgagag ctccccggac aaacggctca
601ctctgtccca gatctacgag tggatggtgc gttgcgtgcc ctacttcaag gataagggcg
661acagcaacag ctctgccggc tggaagaact ccatccggca caacctgtca ctgcatagtc
721gattcatgcg ggtccagaat gagggaactg gcaagagctc ttggtggatc atcaaccctg
781atggggggaa gagcggaaaa gccccccggc ggcgggctgt ctccatggac aatagcaaca
841agtataccaa gagccgtggc cgcgcagcca agaagaaggc agccctgcag acagcccccg
901aatcagctga cgacagtccc tcccagctct ccaagtggcc tggcagcccc acgtcacgca
961gcagtgatga gctggatgcg tggacggact tccgttcacg caccaattct aacgccagca
1021cagtcagtgg ccgcctgtcg cccatcatgg caagcacaga gttggatgaa gtccaggacg
1081atgatgcgcc tctctcgccc atgctctaca gcagctcagc cagcctgtca ccttcagtaa
1141gcaagccgtg cacggtggaa ctgccacggc tgactgatat ggcaggcacc atgaatctga
1201atgatgggct gactgaaaac ctcatggacg acctgctgga taacatcacg ctcccgccat
1261cccagccatc gcccactggg ggactcatgc agcggagctc tagcttcccg tataccacca
1321agggctcggg cctgggctcc ccaaccagct cctttaacag cacggtgttc ggaccttcat
1381ctctgaactc cctacgccag tctcccatgc agaccatcca agagaacaag ccagctacct
1441tctcttccat gtcacactat ggtaaccaga cactccagga cctgctcact tcggactcac
1501ttagccacag cgatgtcatg atgacacagt cggacccctt gatgtctcag gccagcaccg
1561ctgtgtctgc ccagaattcc cgccggaacg tgatgcttcg caatgatccg atgatgtcct
1621ttgctgccca gcctaaccag ggaagtttgg tcaatcagaa cttgctccac caccagcacc
1681aaacccaggg cgctcttggt ggcagccgtg ccttgtcgaa ttctgtcagc aacatgggct
1741tgagtgagtc cagcagcctt gggtcagcca aacaccagca gcagtctcct gtcagccagt
1801ctatgcaaac cctctcggac tctctctcag gctcctcctt gtactcaact agtgcaaacc
1861tgcccgtcat gggccatgag aagttcccca gcgacttgga cctggacatg ttcaatggga
1921gcttggaatg tgacatggag tccattatcc gtagtgaact catggatgct gatgggttgg
1981attttaactt tgattccctc atctccacac agaatgttgt tggtttgaac gtggggaact
2041tcactggtgc taagcaggcc tcatctcaga gctgggtgcc aggctgccca actttcttgt
2101acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2161gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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