Construct: ORF ccsbBroad304_00578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000998.1_s304c1, BRDN0000403210
- Derived from:
- ccsbBroadEn_00578
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLI1 (2313)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00578
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | NM_002017.5 | 100% | 100% | |
| 2 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | NM_001167681.2 | 92.6% | 92.6% | 0_1ins99 |
| 3 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | XM_011542701.2 | 92.6% | 92.6% | 0_1ins99 |
| 4 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | XM_017017405.1 | 92.6% | 92.6% | 0_1ins99 |
| 5 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | XM_017017406.1 | 92.6% | 92.6% | 0_1ins99 |
| 6 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | XM_011542702.1 | 90.7% | 90.7% | 653_654ins126 |
| 7 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | NM_001271010.1 | 84.5% | 82.5% | (many diffs) |
| 8 | human | 2313 | FLI1 | Fli-1 proto-oncogene, ETS t... | NM_001271012.1 | 56.8% | 56.4% | (many diffs) |
| 9 | mouse | 14247 | Fli1 | Friend leukemia integration 1 | NM_008026.5 | 90.9% | 96.6% | (many diffs) |
| 10 | mouse | 14247 | Fli1 | Friend leukemia integration 1 | XM_006510008.2 | 84.2% | 89.3% | (many diffs) |
| 11 | mouse | 14247 | Fli1 | Friend leukemia integration 1 | XM_006510009.2 | 84.2% | 89.3% | (many diffs) |
| 12 | mouse | 14247 | Fli1 | Friend leukemia integration 1 | XM_017313133.1 | 84.2% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1422
- ORF length:
- 1356
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgggactatt aaggaggctc tgtcggtggt gagcgacgac cagtccctct 121 ttgactcagc gtacggagcg gcagcccatc tccccaaggc cgacatgact gcctcgggga 181 gtcctgacta cgggcagccc cacaagatca accccctccc accacagcag gagtggatca 241 atcagccagt gagggtcaac gtcaagcggg agtatgacca catgaatgga tccagggagt 301 ctccggtgga ctgcagcgtt agcaaatgca gcaagctggt gggcggaggc gagtccaacc 361 ccatgaacta caacagctat atggacgaga agaatggccc ccctcctccc aacatgacca 421 ccaacgagag gagagtcatc gtccccgcag accccacact gtggacacag gagcatgtga 481 ggcaatggct ggagtgggcc ataaaggagt acagcttgat ggagatcgac acatcctttt 541 tccagaacat ggatggcaag gaactgtgta aaatgaacaa ggaggacttc ctccgcgcca 601 ccaccctcta caacacggaa gtgctgttgt cacacctcag ttacctcagg gaaagttcac 661 tgctggccta taatacaacc tcccacaccg accaatcctc acgattgagt gtcaaagaag 721 acccttctta tgactcagtc agaagaggag cttggggcaa taacatgaat tctggcctca 781 acaaaagtcc tccccttgga ggggcacaaa cgatcagtaa gaatacagag caacggcccc 841 agccagatcc gtatcagatc ctgggcccga ccagcagtcg cctagccaac cctggaagcg 901 ggcagatcca gctgtggcaa ttcctcctgg agctgctctc cgacagcgcc aacgccagct 961 gtatcacctg ggaggggacc aacggggagt tcaaaatgac ggaccccgat gaggtggcca 1021 ggcgctgggg cgagcggaaa agcaagccca acatgaatta cgacaagctg agccgggccc 1081 tccgttatta ctatgataaa aacattatga ccaaagtgca cggcaaaaga tatgcttaca 1141 aatttgactt ccacggcatt gcccaggctc tgcagccaca tccgaccgag tcgtccatgt 1201 acaagtaccc ttctgacatc tcctacatgc cttcctacca tgcccaccag cagaaggtga 1261 actttgtccc tccccatcca tcctccatgc ctgtcacttc ctccagcttc tttggagccg 1321 catcacaata ctggacctcc cccacggggg gaatctaccc caaccccaac gtcccccgcc 1381 atcctaacac ccacgtgcct tcacacttag gcagctacta ctacccaact ttcttgtaca 1441 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1501 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta